ratio of z to xy extension) is determined using a reference dye. This is the prerequisite to determine correc... "Absolute Diffusion Coefficients: Compilation of Reference Data for FCS Calibration" available from PicoQuan... Tutorial =====
==== Select a file and generate a reference FCS curve ====
* Start [[products:SymPhoTime64... n constant is chosen as calibration value. If the reference were the concentration, the assignment can be cha
me flat.
==== Objective Types ====
An excellent reference for the subject is given in [[http://pubs.acs.o... rature.
===== Further Reading =====
**Note:** The references given here serve as examples. There are many mor... "Accurate Diffusion Coefficients of Organosoluble Reference Dyes in Organic Media Measured by Dual-Focus Fluo
energy transfer (FRET)-based picosecond lifetime reference for instrument response evaluation \\
Measuremen... isional quenching of Erythrosine B as a potential reference dye for impulse response function evaluation\\
A... ichard D Ludescher, Ignacy Gryczynski\\
Standard reference for instrument response function in fluorescence
"Absolute Diffusion Coefficients: Compilation of Reference Data for FCS Calibration" available from PicoQuan... ur system regularly for correct alignment using a reference dye as
ATTO488.\\
In this tutorial, we use the de
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* In the pop-up window, select the reference-measurement (in the example select //''non-FRET s... Note:** This will import the overall decay of the reference measurement. Make sure the measurement contains o