{{tag>howto analysis FLIM imaging tutorial SymPhoTime}}
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====== Lifetime Fitting Using the FLIM Analysis ======
===== Summary =====
This tuto... hows step-by-step, how the FLIM analysis of SymPhoTime 64 can be used to perform a lifetime fit and how to extract and read the results. In detail, the FLIM a
g>tutorial howto analysis FLIM imaging FRET SymPhoTime}}
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====== FLIM-FRET Calculation for M...
This tutorial shows step-by-step, how the "Lifetime FRET Image" analysis of SymPhoTime 64 can be used to calculate pixel-by-pixel the average [[glossary:fr... average FRET-efficiency, first the donor only lifetime is determined using cells that have only been tra
{{tag>howto tutorial FLIM analysis SymPhoTime MT LSM_upgrade reconvolution fitting}}
====== Lifetime-Fitting Using the Rapid Reconvolution Model =====... IM]].
Here in detail the FLIM analysis of SymPhoTime 64 is used to perform a lifetime fit and how to extract and read the results. A mixture of Dragon Green
{{tag> tutorial howto time-trace analysis SymPhoTime}}
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======Intensity Time Trace Analysis======
===== Summary =====
This tutorial shows step-by-step, how to draw intensity time traces from a single molecule. The script require
{{tag>howto tutorial FLIM analysis SymPhoTime MicroTime LSM_upgrade}}
====== Lifetime-Fitting Using the FLIM Analysis (updated for SymPhoTime V 2.5 and above) ======
===== Introduction ====
{{tag>howto analysis lifetime tutorial SymPhoTime}}
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====== SymPhoTime Lifetime Fitting ======
===== Summary =====
This tutorial shows step-by-step, how to fit a selecte
g>tutorial howto FRET FLIM imaging analysis SymPhoTime}}
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====== FLIM-FRET Calculation for S...
This tutorial shows step-by-step, how the "Lifetime FRET Image" analysis of [[products:SymPhoTime64|SymPhoTime 64]] can be used to calculate pixel-by-pixel the FRET efficiency in an image containing a
ging analysis dynamics tutorial LSM_upgrade SymPhoTime}}
====== Visualizing Dynamics Using the Multi Frame FLIM Analysis (updated for SymPhoTime v2.5 and above) ======
===== Introduction =====
... age containing many frames is treated here like a time-series. The example image shows the plasma membra... rane tension changes through its fluorescence lifetime changes. This has been published 2018 in nature:
g> howto FLIM imaging ROI analysis tutorial SymPhoTime}}
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====== ROI Fitting Using the FLIM A... [[glossary:flim|FLIM]] script of [[products:SymPhoTime64|SymPhoTime 64]] can be used to fit several regions of interest (ROIs), and how to extract and interpr... start the script ====
* Start [[products:SymPhoTime64|SymPhoTime 64]] software.
* Open the "Samples
{{tag>Howto Analysis Tutorial SymPhoTime Tips&Tricks}}
====== SymPhoTime 64 Analysis Tips and Tricks ======
~~TOC~~
===== Summary =====
Th... orial explains a few features of [[products:SymPhoTime64]] that can help to make working with the softwa... * Different file-types used or generated by SymPhoTime64
* Image Display
* ROI-handling
* Export
{{tag>howto tutorial FLIM analysis SymPhoTime MT LSM_upgrade}}
====== ROI Fitting Using the FLIM Analysis (updated for SymPhoTime v2.5)======
===== Introduction =====
This tutoria... shows step-by-step, how the FLIM script of SymPhoTime 64 can be used to fit several regions of interest... file and start the analysis ====
* Start SymPhoTime 64 software (this tutorial uses version 2.5, ther
ti-frame imaging analysis dynamics tutorial SymPhoTime}}
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====== Visualizing Dynamics Using t... start the script ====
* Start [[products:SymPhoTime64|SymPhoTime 64]] software.
* Open the "Samples" workspace via "File -> Open Workspace" from the main... e "Samples" workspace is delivered with the SymPhoTime 64 and on the CD-ROM and contains example data to
{{tag> howto tutorial FCS FLCS analysis SymPhoTime}}
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====== Separation of 2 Species with Different Lifetimes Using FLCS ======
===== Summary =====
This t... e of ATTO655 and Cy5 based on their different lifetimes.
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===== Backgro... . cis/trans isomerization.\\
The fluorescence lifetime of both dyes differ significantly (~1.8 ns for AT
{{tag> tutorial howto FRET PIE analysis time-trace SymPhoTime}}
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====== Calculate Ratiometric Single Pair FRET Distributions Using PIE-FRE... cript requires a spectrally resolved fluorescence time trace, in which donor and acceptor have been exci... gJpZhrY?large }}
**Note:** The script requires a time trace containing the fluorescence of two spectral
{{tag> tutorial howto time-trace analysis FRET SymPhoTime}}
~~NOTOC~~
====== Calculate Ratiometric Single Pair FRET Distributions... cript requires a spectrally resolved fluorescence time trace. As a result, the distribution of FRET effi... requisites =====
**Note:** The script requires a time trace containing the fluorescence of two spectral