g>tutorial howto analysis FLIM imaging FRET SymPhoTime}}
~~NOTOC~~
====== FLIM-FRET Calculation for M...
This tutorial shows step-by-step, how the "Lifetime FRET Image" analysis of SymPhoTime 64 can be used to calculate pixel-by-pixel the average [[glossary:fr... average FRET-efficiency, first the donor only lifetime is determined using cells that have only been tra
ial =====
==== Microscopy ====
[[software:SymPhoTime64]] \\
A complete software suite for acquisition and analysis of time resolved fluorescence data. SymPhoTime features a powerful scripting language that allows customisation... sis is currently not included in [[products:SymPhoTime64]]. However, you can use [[http://www.lfd.uci.ed
ved.
Such a cocktail cannot be stored for a long time. Latest the KI will photochemically decompose.
=... of course) any of the above mentioned fast decay time fluorophores and record their response. The signa... surement series, provided that the system has had time to warm up (~15min), and neither the repetition r... used as for the sample), and re-used later. Over time or upon changes of the excitation wavelength, the
{{tag>howto analysis lifetime tutorial SymPhoTime}}
~~NOTOC~~
====== SymPhoTime Lifetime Fitting ======
===== Summary =====
This tutorial shows step-by-step, how to fit a selecte
etectors slightly.
===== Antibunching using SymPhoTime =====
*with [[Products:PicoHarp 300]] in [[g... ossary:T3-mode]]
*not possible with SymPhoTime (32-bit version), because in SPT32 the Sync Divid... 400]] softwares as well as with [[software:SymPhoTime64]], where the sync divider can be disabled.
Usi... *measurements are possible with [[software:SymPhoTime64]]
==== Changing from Normal Acquisition (T3-mo
raphy|config option]].
===== Quoting =====
Some times you want to mark some text to show it's a reply ... 1 Col 3 |
^ Heading 4 | no colspan this time | |
^ Heading 5 | Row 2 Col... Col 3 |
^ Heading 4 | no colspan this time | |
^ Heading 5 | Row 2 C... locks described in the previous section, but this time the name of the language syntax to be highlighted
{{tag>howto tutorial FLIM analysis SymPhoTime MicroTime LSM_upgrade}}
====== Lifetime-Fitting Using the FLIM Analysis (updated for SymPhoTime V 2.5 and above) ======
===== Introduction ====
g> howto FLIM imaging ROI analysis tutorial SymPhoTime}}
~~NOTOC~~
====== ROI Fitting Using the FLIM A... [[glossary:flim|FLIM]] script of [[products:SymPhoTime64|SymPhoTime 64]] can be used to fit several regions of interest (ROIs), and how to extract and interpr... start the script ====
* Start [[products:SymPhoTime64|SymPhoTime 64]] software.
* Open the "Samples
{{tag>howto tutorial FLIM analysis SymPhoTime MT LSM_upgrade}}
====== ROI Fitting Using the FLIM Analysis (updated for SymPhoTime v2.5)======
===== Introduction =====
This tutoria... shows step-by-step, how the FLIM script of SymPhoTime 64 can be used to fit several regions of interest... file and start the analysis ====
* Start SymPhoTime 64 software (this tutorial uses version 2.5, ther
{{tag>SymPhoTime MicroTime200 Scanner PI Physik_Instrumente E725 E710 MadCityLabs Mad_City_Labs PC}}
~~TOC~~
====== Su... ted MT200 PC and Hardware Configuration for SymPhoTime 64 ======
**NOTE:** When upgrading your system w... * WinXP: NO
=====Supported Hardware (for MicroTime200):=====
====Harps (TCSPC units)====
===PicoHa