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- how_to_measure_the_instrument_response_function_irf @howto
- {{tag>howto IRF acquisition video Nikon LSM_upgrade}} ~~TOC~~ ====== How to Measure the Instrument Response Function (IRF) ====== (For an explanation of the term Instrument Response Function see [[glossary:IRF]]) The following video shows how to measure na IRF on confocal microscopes. {{youtube>cjSgCBe3ReM?la
- how_to_work_with_the_instrument_response_function_irf @howto
- {{tag>howto IRF tutorial analysis MicroTime LSM_upgrade}} ====== How to Work with Instrument Response... h a Microscope ====== ===== Introduction ===== IRF stands for Instrument Response Function, and it r... rument contribute to both shape and width of the IRF. This width is an indicator the timing resolution of the instrument. {{ howto:irf.png?500 |}} The IRF width is approx. a quadrati
- flim_fret_calculation_for_multi_exponential_donors @howto
- estimation of the instrument response function (=IRF) is shown. The IRF reconstruction is deducted from the rising edge of the TCSPC histogram. * As dat... } **Response:**\\ * In the TCSPC window, the IRF is displayed in bright red, the data fitting limi... C window. * The new fitting parameters "Shift IRF" and "Bkgr IRF" (=background IRF) appear in the f
- lifetime-fitting_using_the_flim_script @howto
- estimation of the instrument response function (=IRF) is shown. The IRF reconstruction is deducted from the rising edge of the [[glossary:TCSPC]] histogram... 00 |}} ** Response:**\\ In the TCSPC window, the IRF is displayed in bright red, the data fitting limi... TCSPC window.\\ The new fitting parameters "Shift IRF" and "Bkgr IRF" (=background IRF) appear in the f
- lifetime_fitting_using_the_flim_analysis @howto
- estimation of the instrument response function (=IRF) is shown. The IRF reconstruction is deducted from the rising edge of the TCSPC histogram. * To enh... } **Response:**\\ * In the TCSPC window, the IRF is displayed in bright red, the data fitting limi... C window. * The new fitting parameters "Shift IRF" and "Bkgr IRF" (=background IRF) appear in the f
- visualizing_dynamics_using_the_multiframe-flim_script @howto
- estimation of the instrument response function (=IRF) is shown. The IRF reconstruction is deducted from the rising edge of the [[glossary:TCSPC]] histogram... 00 |}} ** Response:**\\ In the TCSPC window, the IRF is displayed in bright red, the data fitting limi... TCSPC window.\\ The new fitting parameters "Shift IRF" and "Bkgr IRF" (=background IRF) appear in the f
- irf @glossary
- ====== IRF ====== IRF stands for **I**nstrument **R**esponse **F**unction. The IRF is the best approximation to a temporally infinit... itation is the most intuitive contribution to the IRF. Of course, the temporal resolution of all compon
- differential_count_rate @glossary
- {{tag> pile-up dead_time IRF differential_countrate}} ====== Differential Count Rate ====== Ideally, a decay curve and the corresponding IRF are recorded at the same //differential count rat... to avoid the [[pile-up effect]]). However, for an IRF measurement the same "low" average count rate can be far too high. Consider a 150 ps FWHM IRF measured at an average count rate of 100 kcps (ki
- lifetime_fitting_using_the_tcpsc_fitting_script @howto
- del. **Response:** * In the TCSPC window, the IRF is displayed in bright red and the data fitting l... C window. * The new fitting parameters "Shift IRF" and "Bkgr IRF" (=background IRF) appear in the fitting parameter table. {{ lifetime_fitting_using_the_tcpsc_fitting_
- roi_fitting_using_the_flim_script @howto
- estimation of the instrument response function (=IRF) is shown. The IRF reconstruction is deducted from the rising edge of the images TCSPC histogram. *... } **Response:**\\ * In the TCSPC window, the IRF is displayed in bright red, the data fitting limi... C window. * The new fitting parameters "Shift IRF" and "Bkgr IRF" (=background IRF) appear in the f
- update @howto
- estimation of the instrument response function (=IRF) is shown. The IRF reconstruction is deducted from the rising edge of the [[glossary:TCSPC]] histogram... 00 |}} ** Response:**\\ In the TCSPC window, the IRF is displayed in bright red, the data fitting limi... TCSPC window.\\ The new fitting parameters "Shift IRF" and "Bkgr IRF" (=background IRF) appear in the f
- data_file_import @howto
- o:how_to_measure_the_instrument_response_function_irf|Instrument Response Function (IRF)]] and a decay curve of Coumarin6 dissolved in ethanol. During file ... Original data file content }} Trace #0 shows the IRF, Trace #2 is the decay curve. The other data bloc... any more histograms. It is also possible that an IRF measurement and a decay measurement are stored in
- lifetime-fitting_using_the_rapid_reconvolution_algorithm @howto
- estimation of the instrument response function (=IRF) is shown. The IRF reconstruction is deducted from the rising edge of the [[glossary:TCSPC]] histogram... ill a bit of deviation in the residuals after the IRF in the decay window. This however can be explaine... unchecking them in the fit table, as well as the IRF background and IRF Fit. * One the single pixel
- reconvolution_fit @howto
- an ''.etc'' container that contains at least one IRF histogram and at least one decay curve. Open the ... , we need to select and then assign a curve as an IRF and then change the fitting modality. {{ :howto:e... (selecting) ''crv[0]'', the first histogram, the IRF in this particular case, updates the plot. Now, a... the "Curve type" can be changed from "Decay" to "IRF". {{ :howto:et_fitwindow2.png |}} \\ \\ The p
- measurement_hardware_instrumentation
- o:how_to_measure_the_instrument_response_function_irf|How to measure the Instrument Response function (IRF)]] */ [[Some origins of multiexponetial decays f... o:how_to_measure_the_instrument_response_function_irf|How to measure the Instrument Response function (IRF)]] [[howto:FLIM Measurement using a Nikon A1 wit