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calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script @howto
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ratio of z to xy extension) is determined using a reference dye. This is the prerequisite to determine correc... "Absolute Diffusion Coefficients: Compilation of Reference Data for FCS Calibration" available from PicoQuan... Tutorial ===== ==== Select a file and generate a reference FCS curve ==== * Start [[products:SymPhoTime64... n constant is chosen as calibration value. If the reference were the concentration, the assignment can be cha
how_to_measure_the_instrument_response_function_irf @howto
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energy transfer (FRET)-based picosecond lifetime reference for instrument response evaluation \\ Measuremen... isional quenching of Erythrosine B as a potential reference dye for impulse response function evaluation\\ A... ichard D Ludescher, Ignacy Gryczynski\\ Standard reference for instrument response function in fluorescence
fluorescence_correlation_spectroscopy-_a_short_introduction @general
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me flat. ==== Objective Types ==== An excellent reference for the subject is given in [[http://pubs.acs.o... "Accurate Diffusion Coefficients of Organosoluble Reference Dyes in Organic Media Measured by Dual-Focus Fluo
calculate_and_fit_fcs_traces_with_the_fcs_script @howto
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"Absolute Diffusion Coefficients: Compilation of Reference Data for FCS Calibration" available from PicoQuan... ur system regularly for correct alignment using a reference dye as ATTO488.\\ In this tutorial, we use the de
pattern_matching @howto
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e_23.png }} * In the pop-up window, select the reference-measurement (in the example select //''non-FRET s... Note:** This will import the overall decay of the reference measurement. Make sure the measurement contains o
measurement_hardware_instrumentation
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5232/kundenposter_lsmmt200_final_lowres.pdf|Quick Reference for Confocal Time-resolved Microscopy (FLIM, FRET
community_news @general
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elected. \\ \\ * **31.03.20** Extremely helpful reference paper about confocal microscopy. [[https://www.na
antibunching_measurements @howto
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e see [[Diamond NV centers]] *depending on preference set laser repetition rate in order to allow full
using_the_antibunching_script @howto
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the photon pairs, detector 1 or 2 can be used as reference. The green curve is the average of both curves.