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- separation_of_2_species_with @howto
- calculate separate autocorrelation curves for the two components of a mixture of ATTO655 and Cy5 based ... large }} ===== Background Information ===== The two fluorophores ATTO655 (ATTO-Tec, Germany) and Cy5 ... nt. The aim of this script is to demonstrate, how two FCS traces are calculated from the same dataset, ... In this file, the light was evenly splitted onto two detectors. Therefore, select both detectors in th
- syntax @wiki
- orce a newline** without a paragraph, you can use two backslashes followed by a whitespace or the end o... is some text with some linebreaks\\ Note that the two backslashes are only recognized at the end of a l... some text with some linebreaks\\ Note that the two backslashes are only recognized at the end of a l... lists. To create a list item, indent your text by two spaces and use a ''*'' for unordered lists or a '
- calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script @howto
- uires a time trace containing the fluorescence of two spectrally separated channels, one channel mainly... ) analysis of the script, pulsed excitation using two lasers is required, one laser exciting the donor,... aser driver PDL828 “Sepia II” in combination with two pulsed diode lasers. \\ ===== Step-by-Step Tut... ngs" unchanged and press "Calculate". **Note:** Two channels need to be active in order to calculate
- pattern_matching @howto
- 600 }} **Response:** The image now clearly shows two populations of different lifetimes. This means, that probably two patterns are sufficient to fit the image. **Note... ing_Image_4.png?600 }} **Note:** In the example, two peaks in the average lifetime (x-axis) can alread... Zoom into the //Decay Diversity Map// to see the two different populations and their different lifetim
- flim_fret_calculation_for_multi_exponential_donors @howto
- xample image used is an image showing four cells, two of them only transfected with the CENP-B-Cerulean complex, the other two transfected with both constructs. Therefore, the ... ted. **Note:**\\ Now four cells are visible, the two nuclei on the left have longer Fast lifetimes then the other two. These are the cells transfected only with the do
- fluorescence_correlation_spectroscopy-_a_short_introduction @general
- ings). In <imgref figure1>A and <imgref figure1>B two different scenarios are illustrated. In <imgref f... introduced (listing probably not complete): * **Two-color fluorescence cross-correlation spectroscopy... lysis of proteome dynamics in living cells" * **two-photon FCS** \\ Schwille and Heinze, 2001, "Two-Photon cross-correlation spectroscopy" * **scanning
- calculate_ratiometric_single_pair_fret_distributions @howto
- uires a time trace containing the fluorescence of two spectrally separated channels, one channel mainly... ings" unchanged and press "Calculate". **Note:** Two channels need to be active in order to calculate ... ter mainly excited via FRET - was detected in the two different channels. In this trace, donor and acce... were recorded with a slight time delay, therefore two peaks can be seen in the green marked area. This
- lifetime-fitting_using_the_rapid_reconvolution_algorithm @howto
- each of these parameters individually. There are two ways to define the new threshold. You can use the... ount rates there is an increasing likelihood that two or more such detector pulses will overlap and mer... constant of the systematic errors. * Try last two steps with the ROI 1. {{ howto:monoexpo_roi1.pn... on is not sufficient. Therefore, we carry on with two components. {{ howto:biexpo_roi1.png |}} **
- using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs @howto
- (FLCCS) curve from a measurement of a mixture of two labeled oligunucleotides moving independently fro... ethod to determine molecular interactions between two molecules labeled with different dyes. Usually, the dyes attached to the two molecules differ in their absorption and emission... oomlight.\\ Therefore, it is possible to generate two patterns: one for the fluorescence response after
- data_file_import @howto
- native TimeHarp260 software]]. This file contains two histograms: an [[howto:how_to_measure_the_instrum... measurement and a decay measurement are stored in two separate ''.phu'' files. This is no problem, but ... data container: {{ :howto:et2_workspace2.png |}} Two histograms have been imported. They are shown as ... \ \\ Unlike ''Decay_Coumarin_6.phu'' containing two histograms, both ''Anthracene_EtOH.phu'' and ''IR
- intensity_time_trace_analysis @howto
- , its fluorescence was split by polarization onto two detectors. {{ using_the_intensity_time_trace_scr... Image_5.png?600 }} **Note:** The window contains two different regions:\\ - **Left:** Plotting optio... nse:** As activated under "Select data channels", two intensity plots are calculated and plotted according to the two selected detector channels. {{ using_the_intensit
- measurement_hardware_instrumentation
- l width analysis]] [[video_tutorials#Kurt Thorn: Two Photon Microscopy| Two Photon Microscopy (TPM, TPE) by Kurt Thorn]] [[general:advantages_and_disadvantages_of_two_photon_excitation_tpe|Advantages and disadvantages of Two Photon Excitation (TPE)]] [[howto:how_to_measure
- video_tutorials
- niques/kurt-thorn-part-1.html]] ==== Kurt Thorn: Two Photon Microscopy ==== Kurt Thorn of UCSF talks about two-photon microscopy which uses intense pulsed laser... p://www.ibiology.org/ibioeducation/taking-courses/two-photon-microscopy.html]] ==== Philippe Bastiaen... d as an imaging technique in confocal microscopy, two-photon excitation microscopy, and multiphoton tom
- calculate_ratiometric_fret-images @howto
- fret.pdf]].\\ The example image shows four cells, two of them were only transfected with CENP-B-Cerulean, the other two contain both constructs. The same image is also u... culate". **Response:**\\ * Automatically, the two active detector channels are assigned to the dono... sfected with the donor construct, while the other two cells are transfected with both constructs. The f