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- create_time_gated_image
- ow with the header "Set Time Gate" {{:flim-image_after_loading.png|}} * Double click on this green wi... s the selection is not so precise. {{:flim-image_after_gate_selection_1.png|}} * Click "Calculate Fas... e time gate on the imaging photons {{:flim-image_after_gate_selection_2.png|}}}} * The image is updat... you can adjust the rainbow scale. {{:flim-image_after_gate_selection_3.png|}} * To get a pure time-ga
- calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script @howto
- . The second area shows the fluorescence response after the second pulse (at 635 nm), which was used to e... ph is automatically assigned to the donor channel after donor excitation (detector 2). The second graph shows the acceptor trace after donor excitation – automatically assigned to channel 1. The third graph shows the acceptor trace after acceptor excitation, and the last graph shows the
- symphotime_tips_and_tricks @howto
- e acquisition (you cannot change this information after the acquisition) More detailed information can b... y inside the ''.ptu'' file and cannot be modified after the measurement (see <imgref image1>). ==== Re... </WRAP>| * some parameters are only available after performing a FLIM-Fit (i.e. the different lifetim... "FastLT" along the line is displayed. \\ {{:howto:after_selection_line_profile.jpg?350|}} Note: The
- fluorescence_correlation_spectroscopy-_a_short_introduction @general
- sity F at time t with the fluuorescence intensity after a lag time τ divided by the mean fluorescence int... nfocal volume can be determined ($\ref{eqVeff}$). After the calibration of the detection volume one can u... robability of having the same level of the signal after lag time, $t=t_o+\tau$. The main difference of FC
- lifetime-fitting_using_the_rapid_reconvolution_algorithm @howto
- etime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwise, inte... here is still a bit of deviation in the residuals after the IRF in the decay window. This however can be ... oi1.png |}} * If you compare the result image after the fit with the fast lifetime image above, you w
- roi_fitting_using_the_flim_script @howto
- etime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwise, inte... a second array encodes the Fast Lifetime values. After removal of the header and the unwanted array, ind... ROI 2\\ * Keep the cursor over the graph, and after a right mouse click, select from the context menu
- using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs @howto
- e two patterns: one for the fluorescence response after pulsed excitation (e.g. for the green fluorescent dye) and one for the fluorescence response after cw excitation.\\ After these TCSPC patterns have been generated, the green spectral channel using the pu
- visualizing_dynamics_using_the_multiframe-flim_script @howto
- etime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwise, inte... ponse:**\\ The average lifetime, you can see that after adding the sugar, has been dropped gradually abou... more arrays depending on the fitting model used. After removal of the header and the unwanted array, ind
- pile-up_effect @glossary
- s and [[TCSPC]] electronics have a [[dead time]]. After registering one photon the device needs some time... ering the dead time we can detect only one photon after one laser shot. "Pile up" describes the effects o
- data_file_import @howto
- ll end up in dedicated ''.etc'' data containers. After importing ''Decay_Coumarin_6.phu'', the workspace... ike this: {{ :howto:et2_workspace1.png |}} \\ \\ After expanding the data container: {{ :howto:et2_works
- flim_fret_calculation_for_multi_exponential_donors @howto
- etime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwise, inte... etime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwise, inte
- how_to_measure_the_instrument_response_function_irf @howto
- torage: <code> keep at ~ 4°C, renew the solution after one month </code> Spectra: <code> excitation fr... ctrometers, the IRF is usually measured before or after each measurement. In microscopy-applications, th
- lifetime_fitting_using_the_flim_analysis @howto
- etime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwise, inte... he pixel decay in grey, the background before and after the decay is practically 0.Therefore, we can also
- lifetime-fitting_using_the_flim_script @howto
- etime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwise, inte... he pixel decay in grey, the background before and after the decay is practically 0.Therefore, we can also
- measuring_quantum_yield @howto
- tart Optimization”. {{ :howto:5.png?600 |}} * After the optimization has successfully finished, click... the “Start” button. {{ :howto:9.png?600 |}} * After the acquisition of the blank spectrum has finishe