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calculate_and_fit_fcs_traces_with_the_fcs_script @howto

6 Hits, Last modified: 9 years ago

* The first decreasing part is due to detector afterpulsing and an unwanted artifact from the detector. If cw... png?600 }} **Note:** A possibility to remove the afterpulsing when working with cw excitation is splitting the ... mainly [[glossary:Hybrid PMT]]s) also have so low afterpulsing that the problem does not occur. * If the samp... ith a pulsed laser, there's another way to remove afterpulsing: * Open the "FLCS Background Correction" dropdo

calculate_fccs_trace_with_the_grouped_fcs_script @howto

2 Hits, Last modified: 5 years ago

g part of the autocorrelations is due to detector afterpulsing, an unwanted artifact from the detector. As the afterpulsing is not correlated between both detectors, the cros

calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script @howto

2 Hits, Last modified: 6 years ago

e background in the TCPSC window. As the detector afterpulsing is not correlated with the laser pulse on the time range of interest, photons caused by detector afterpulsing behave similar as background photons. * Click o

differential_count_rate @glossary

1 Hits, Last modified: 8 years ago

hape) but also an increased probability to detect afterpulsing. A frequently asked question is: "//Why we have

separation_of_2_species_with @howto

1 Hits, Last modified: 11 years ago

ground, mainly caused by dark counts and detector afterpulsing. {{ separation_of_2_species_with_Image_32.png?6