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advantages_and_disadvantages_of_two_photon_excitation_tpe @general
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*The excitation is much broader so that many dyes are excited at the same time. This is an advantag... background. *The molecular brightness of the dyes is generally around one order of magnitude smalle... cule applications. * The photobleaching of the dyes is much more pronounced. This again limits the nu... of fluorescence photons you can achieve from your dyes. Bleaching only takes place in the excitation vol
separation_of_2_species_with @howto
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nstant is too similar to distinguish between both dyes in a mixture by means of standard FCS.\\ Nevertheless, the autocorrelation curves of both dyes differ in the microsecond range. ATTO655 shows pr... somerization.\\ The fluorescence lifetime of both dyes differ significantly (~1.8 ns for ATTO655 and ~1 ... s to define these filters: * If the different dyes have been measured separately, and TCPSC curves o
calculate_and_fit_fcs_traces_with_the_fcs_script @howto
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* **Note** that the diffusion coefficients of all dyes are temperature dependent. * FCS measurements a... e through the confocal volume. Examples for these dyes are ATTO655 or ATTO425. Also dyes with a moderate triplet contribution can be fitted with this model if... cell membrane is observed. This model is made for dyes moving within a plane, e.g. modified lipids in a
using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs @howto
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ions between two molecules labeled with different dyes. Usually, the dyes attached to the two molecules differ in their absorption and emission properties but... abeled with both fluorophores.\\ FCCS calibration dyes are available e.g. from IBA ([[http://www.iba-lif... ally, a positive control is needed, in which both dyes are moving in a coordinated fashion.\\ Additional
calculate_fccs_trace_with_the_grouped_fcs_script @howto
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ions between two molecules labeled with different dyes. Usually, the dyes attached to the two molecules of interest differ in their absorption and emission pr... abeled with both fluorophores.\\ FCCS calibration dyes are available e.g. from IBA ([[http://www.iba-lif
measurement_hardware_instrumentation
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Some origins of multiexponetial decays for single dyes|Some origins of multiexponetial decays for pure dyes]] ===== Microscopy ===== [[http://www.picoquant
some_origins_of_multiexponetial_decays_for_single_dyes
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= Some origins of multiexponetial decays for pure dyes ====== The fluorescence lifetime of a dye measu... c angle in a perfectly aligned spectrometer pure dyes in homogeneous media may exhibit a multiexponenti
fluorescence_correlation_spectroscopy-_a_short_introduction @general
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/7353/appnote_diffusioncoefficients.pdf| standard dyes for FCS]] and perform a series of measurements wh... Diffusion Coefficients of Organosoluble Reference Dyes in Organic Media Measured by Dual-Focus Fluoresce
how_to_measure_the_instrument_response_function_irf @howto
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/10.1088/0957-0233/27/2/027001 Pyridinium Styryl Dyes have also been used as IRF standard: Lei Li, Men... lph Jimenez, Jianhua Xu\\ Using Pyridinium Styryl Dyes as the Standards of Time-Resolved Instrument Resp
lifetime_fitting_using_the_tcpsc_fitting_script @howto
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c_fitting_script_Image_17.png?600 }} * If both dyes can be fitted with the same model, click "Fit All... le, it is of course meaningless, as two different dyes were fitted. * Place the mouse over the graph,
calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script @howto
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o distinguish molecules with and without acceptor dyes and to filter the events for signals without acce
calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script @howto
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te.\\ Note that the diffusion coefficients of all dyes are temperature dependent.\\ FCS measurements are
flim_fret_calculation_for_multi_exponential_donors @howto
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TCSPC-window, e.g. 2PE excitation with 80MHz and dyes with a lifetime above ~3ns. For explanation on th
pattern_matching @howto
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on raw data. This allows for separating different dyes or regions of different lifetime/decay-behavior i