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advantages_and_disadvantages_of_two_photon_excitation_tpe

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*The excitation is much broader so that many dyes are excited at the same time. This is an advantag... background. *The molecular brightness of the dyes is generally around one order of magnitude smalle... cule applications. * The photobleaching of the dyes is much more pronounced. This again limits the nu... of fluorescence photons you can achieve from your dyes. Bleaching only takes place in the excitation vol

fluorescence_correlation_spectroscopy-_a_short_introduction

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/7353/appnote_diffusioncoefficients.pdf| standard dyes for FCS]] and perform a series of measurements wh... Diffusion Coefficients of Organosoluble Reference Dyes in Organic Media Measured by Dual-Focus Fluoresce