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calculate_and_fit_fcs_traces_with_the_fcs_script
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{{tag> tutorial howto FCS analysis SymPhoTime}} ~~NOTOC~~ ====== Calculate and Fit FCS Traces with the FCS Script ====== ===== Summary ===== This tutorial shows step-by-step, how an auto... olved in water is calculated and fitted using the FCS script. {{ youtube>NRFaEuSb778?large }} ===== B
calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script
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wto analysis calibration PSF confocal-volume VEFF FCS SymPhoTime}} ~~NOTOC~~ ====== Calibrate the Confocal Volume for and with FCS ====== ===== Summary ===== This tutorial shows... by-step, how to calibrate the confocal volume for FCS measurements with a calibration dye. Therefore, a FCS curve is calculated with the FCS script, that is
calculate_fccs_trace_with_the_grouped_fcs_script
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{{tag> analysis howto tutorial FCS FCCS Cross-Correlation SymPhoTime}} ~~NOTOC~~ ====== Calculate FCCS Traces with the Grouped FCS Script ====== ===== Summary ===== This tutoria... dye-labeled DNA-oligonucleotide using the Grouped FCS script. {{ youtube>0Ht_L3a53E0?large }} ===== B... ngle mouse click. **Note:** This file contains a FCS measurement of a FCCS positive control, a DNA oli
separation_of_2_species_with
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{{tag> howto tutorial FCS FLCS analysis SymPhoTime}} ~~NOTOC~~ ====== Separation of 2 Species with Di... tween both dyes in a mixture by means of standard FCS.\\ Nevertheless, the autocorrelation curves of bo... tically no photophysical fluctuations. Therefore, FCS curves of ATTO655 can be fitted with the simplest... ontrary, exhibits a distinct bunching term on its FCS trace originating from photophysical processes, i
2ffcs
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{{tag> 2fFCS FCS}} ~~TOC~~ ====== 2fFCS ====== 2fFCS is an abbreviation **2 F**ocus **F**luorescence **C**orrelation **S**pectroscopy. Dual
symphotime_tips_and_tricks
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the script with the stored result - For online FCS-results, the calculated molecular brightness can ... result-file opens with a preview display (Image, FCS-traces, TCSPC-curve or Time Trace - depending on ... me]], the image consists of a single frame) === FCS (for each of the max.2 data channels) === <WRAP> <imgcaption CommentFCS| Comment Tab of an FCS online analysis result>{{o
using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs
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{{tag> howto tutorial FCS FLCS crosstalk analysis SymPhoTime}} ~~NOTOC~~ ====== Spectral Crosstalk Rem... tutorial [[Calculate FCCS Trace with the Grouped FCS Script]]. **Note:** This way you can compare the... ngle mouse click. **Note:** This file contains a FCS measurement of a FCCS negative control (two DNA o... lysis" tab and in there, open the drop down menu "FCS". {{ using_the_flcs_script_for_spectral_crosstalk
phasor_analysis
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M data via the Phasor approach. ===== Install SimFCS ===== * Download [[https://www.lfd.uci.edu/globals/ | Globals for Images]] * Install SimFCS according to the documentation: [[http://www.lfd.... t_spt64.png?600 |}} ===== Analysis in Globals/SimFCS ===== * follow the [[https://download.lfd.uc... 9%20-%20Reading%20PicoQuant%20PT3%20files%20for%20FCS%20and%20lifetime%20FCS.pdf| Reading PicoQuant PT3
performing_an_fcs_measurement_with_an_olympus_fv1200_upgrade_kit
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{{tag> howto FCS acquisition video FV1200 Olympus LSM_upgrade}} ====== Performing a FCS measurement with an Olympus FV1200 Upgrade Kit===... } This video is a walkthrough of how to measure a FCS (Fluorescence Correlation Spectroscopy) trace with an Olympus FV1200 upgraded for FLIM and FCS with a PicoQuant upgrade kit. Continue to: {{t
howto
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). ==== General ==== {{topic>tutorial +analysis -FCS -FRET -FLIM -draft &nodate&nouser}} ==== FLIM ==== {{topic>tutorial +analysis +FLIM -FCS -FRET -draft &nodate&nouser}} ==== FRET ==== {{t... al +analysis +FRET -draft &nodate&nouser}} ==== FCS / Correlation ==== {{topic>tutorial +analysis +FCS -draft &nodate&nouser}} ===== EasyTau2 / Analysis
flim_measurement_using_a_nikon_a1_with_a_flim_and_fcs_upgrade
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FLIM Measurement Using a Nikon A1 with a FLIM and FCS Upgrade ====== This tutorial shows the recording... NIS Elements 4.3 integrates control for FLIM and FCS ===== {{ youtube>d_-4MSWznkU?large }} The movie... Kit on a Nikon A1 ]] * [[FLIM-FRET Measurement using an Olympus FV1200 with a FLIM and FCS upgrade]]
determination_of_the_focal_width_with_the_focal
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acement of the beams, which is used in dual focus FCS (2fFCS) measurements.\\ If data are acquired with a scanner controlled by the SymPhoTime 64 software (
mt200fcs
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======MT200 FCS Measurement ====== {{tag>howto MicroTime200 video tutorial}} fluorescence correlation spectroscopy (FCS) is a quantitative measurement of the resolution
antibunching_measurements
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==== Analysis in SymPhoTime ==== *Select FCS Analysis * Select //CrossCorrelation// and /
calculate_ratiometric_single_pair_fret_distributions
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are unsure which binning to select, calculate the FCS curve and check the diffusion time to see the ave
calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script
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flim-fret_measurement_using_an_olympus_fv1200_with_a_flim_and_fcs_upgrade
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intensity_time_trace_analysis
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using_the_antibunching_script
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