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symphotime_tips_and_tricks
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In detail, this tutorial covers: * Different file-types used or generated by SymPhoTime64 * Image... tional options and tools otherwise hidden ===== File-Type Overview ===== ^Workspace: may contain these Filetypes ^ Content of the file ^ How to get such a file in the workspace ^ How to open/process^ |''.ptu''|R
lifetime_fitting_using_the_tcpsc_fitting_script
27 Hits, Last modified:
}} ===== Step-by-Step Tutorial ===== Select a file and start the script * Start [[products:SymPho... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:** Th... e going through this tutorial. **Response:** The files of the sample workspace are displayed in the wor... _fitting_script_Image_1.png }} * Highlight the file ''ATTO655_diff_FLCS-pattern.ptu'' by a single mou
calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script
26 Hits, Last modified:
===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[products:... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:** Th... e going through this tutorial. **Response:** The files of the sample workspace are displayed in the wor... pie-fret_script_Image_1.png }} * Highlight the file ''Cy3+Cy5_diff_PIE-FRET.ptu'' by a single mouse c
calculate_ratiometric_single_pair_fret_distributions
23 Hits, Last modified:
===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[products:S... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:** Th... e going through this tutorial. **Response:** The files of the sample workspace are displayed in the wor... t_distributions_Image_1.png }} * Highlight the file ''Cy3+Cy5_diff_PIE-FRET.ptu'' by a single mouse c
data_file_import
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==== Data file import ==== Create a new workspace or open an existing one: {{ :howto:et2_createopen.png | File menu }} Then select Import Files. A new dialog opens, where the data file is selected: {{ :howto:et2_fileimport.png | Selects the file
separation_of_2_species_with
21 Hits, Last modified:
===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[products:S... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:**\\ ... e going through this tutorial. **Response:** The files of the sample workspace are displayed in the wor... _2_species_with_Image_1.png }} * Highlight the file ''ATTO655+Cy5_diff_FCS+FLCS.ptu'' by a single mou
using_the_anisotropy_image_script
21 Hits, Last modified:
===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[products:S... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:** Th... e going through this tutorial. **Response:** The files of the sample workspace are displayed in the wor... mage_script_Image_1.png?217 }} * Highlight the file ''Cy5_immo_FLIM+Pol-imaging''. {{ using_the_aniso
visualizing_dynamics_using_the_multiframe-flim_script
19 Hits, Last modified:
===== Step-by-Step Tutorial ===== ==== Select a file and start the analysis ==== * Start SymPhoTime... on 2.5). * Open the "Tutorials" workspace via **File -> Open Workspace** from the main menu.\\ **Response:​**\\ ​ The files of the "Tutorials" workspace are displayed in th... e left side of the main window. * Highlight the file ''Hyperosmotic_Shock_MDCK_Cells.ptu'' ​by a singl
flim_fret_calculation_for_multi_exponential_donors
18 Hits, Last modified:
e [[http://www.picoquant.com/images/uploads/page/ files/7267/appnote_flim_fret.pdf]]. The example image ... 4 software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:**\\ ... going through this tutorial. **Response:**\\ The files of the sample workspace are displayed in the wor... onential_donors_Image_1.png }} * Highlight the file ''CENP-labelled _cells_for_FRET.ptu'' by a single
roi_fitting_using_the_flim_script
18 Hits, Last modified:
arge }} ===== Step-by-Step ===== ==== Select a file and start the script ==== * Start [[products:S... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:** Th... e going through this tutorial. **Response:** The files of the sample workspace are displayed in the wor... the_flim_script_Image_1.png }} * Highlight the file ''DaisyPollen_cells_FLIM.ptu'' by a single mouse
using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs
16 Hits, Last modified:
===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[products:S... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:** Th... e going through this tutorial. **Response:** The files of the sample workspace are displayed in the wor... moval_via_flccs_Image_1.png }} * Highlight the file ''IBA488+IBA547_unlinked_mix.ptu'' by a single mo
calculate_fccs_trace_with_the_grouped_fcs_script
15 Hits, Last modified:
===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[products:S... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:** Th... e going through this tutorial. **Response:** The files of the sample workspace are displayed in the wor... calculate_fccs_Image_1.png }} * Highlight the file ''IBA488+547_crosslinked.ptu'' by a single mouse
calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script
14 Hits, Last modified:
[[https://www.picoquant.com/images/uploads/page/files/7353/appnote_diffusioncoefficients.pdf]]). This ... ===== Step-by-Step Tutorial ===== ==== Select a file and generate a reference FCS curve ==== * Star... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:** Th... e going through this tutorial. **Response:** The files of the sample workspace are displayed in the wor
visualizing_dynamics_with_the_multi_frame_flim_analysis
14 Hits, Last modified:
===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[products:S... ] software. * Open the "Samples" workspace via "File -> Open Workspace" from the main menu. **Note:**... e going through this tutorial. **Response:** The files of the sample workspace are displayed in the wor... lizing_dynamics_Image_1.png }} * Highlight the file ''GUVs.ptu'' by a single mouse click. {{ visualiz
intensity_time_trace_analysis
12 Hits, Last modified:
===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[products:S... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:** Th... . to a folder ''C:\SPT_User''. **Response:** The files of the sample workspace are displayed in the wor... me_trace_script_Image_1.png }} * Highlight the file ''Cy5_immo_Lifetime_Trace.ptu'' by a single mouse
using_the_antibunching_script
12 Hits, Last modified:
lifetime-fitting_using_the_flim_script
11 Hits, Last modified:
calculate_and_fit_fcs_traces_with_the_fcs_script
10 Hits, Last modified:
determination_of_the_focal_width_with_the_focal
10 Hits, Last modified:
phasor_analysis
10 Hits, Last modified:
update
10 Hits, Last modified:
calculate_ratiometric_fret-images
9 Hits, Last modified:
flim-fret_calculation_for_single_exponential_donors
9 Hits, Last modified:
lifetime_fitting_using_the_flim_analysis
9 Hits, Last modified:
lifetime-fitting_using_the_rapid_reconvolution_algorithm
9 Hits, Last modified:
howto
6 Hits, Last modified:
registering_new_scripts
5 Hits, Last modified:
how_to_work_with_the_instrument_response_function_irf
4 Hits, Last modified:
pattern_matching
4 Hits, Last modified:
measuring_quantum_yield
1 Hits, Last modified: