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symphotime_tips_and_tricks
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In detail, this tutorial covers: * Different file-types used or generated by SymPhoTime64 * Image... tional options and tools otherwise hidden ===== File-Type Overview ===== ^Workspace: may contain these Filetypes ^ Content of the file ^ How to get such a file in the workspace ^ How to open/process^ |''.ptu''|Raw data in a unified TTTR-f
calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script
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===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[products:... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:** Th... pie-fret_script_Image_1.png }} * Highlight the file ''Cy3+Cy5_diff_PIE-FRET.ptu'' by a single mouse c... Response:** The PIE FRET script is applied to the file ''Cy3+Cy5_diff_PIE-FRET.ptu'' and a new window op
calculate_ratiometric_single_pair_fret_distributions
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===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[products:S... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:** Th... t_distributions_Image_1.png }} * Highlight the file ''Cy3+Cy5_diff_PIE-FRET.ptu'' by a single mouse c... **Response:** The FRET script is applied to the file ''Cy3+Cy5_diff_PIE-FRET.ptu''. Thereby, a new win
using_the_anisotropy_image_script
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===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[products:S... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:** Th... mage_script_Image_1.png?217 }} * Highlight the file ''Cy5_immo_FLIM+Pol-imaging''. {{ using_the_aniso... :** The Anisotropy Image script is applied to the file ''Cy5_immo_FLIM+Pol-Imaging.ptu''. Thereby, a new
lifetime_fitting_using_the_tcpsc_fitting_script
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}} ===== Step-by-Step Tutorial ===== Select a file and start the script * Start [[products:SymPho... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:** Th... _fitting_script_Image_1.png }} * Highlight the file ''ATTO655_diff_FLCS-pattern.ptu'' by a single mou... nse:** The TCSPC Fitting script is applied to the file ''ATTO655_diff_FLCS-pattern.ptu''. Thereby, a new
roi_fitting_using_the_flim_script
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arge }} ===== Step-by-Step ===== ==== Select a file and start the script ==== * Start [[products:S... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:** Th... the_flim_script_Image_1.png }} * Highlight the file ''DaisyPollen_cells_FLIM.ptu'' by a single mouse ... **Response:** The FLIM script is applied to the file ''DaisyPollen_cells_FLIM.ptu''. Thereby, a new Wi
data_file_import
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==== Data file import ==== Create a new workspace or open an existing one: {{ :howto:et2_createopen.png | File menu }} Then select Import Files. A new dialog opens, where the data file is selected: {{ :howto:et2_fileimport.png | Selects the file to be imported }} The file selected above is a s
flim_fret_calculation_for_multi_exponential_donors
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4 software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:**\\ ... onential_donors_Image_1.png }} * Highlight the file ''CENP-labelled _cells_for_FRET.ptu'' by a single... **Response:**\\ The FLIM script is applied to the file ''CENP-labelled_cells_for_FRET.ptu''. Thereby, a ... png }} **Response:**\\ A window opens, where the file containing the IRF can be selected. * Select th
separation_of_2_species_with
15 Hits, Last modified:
===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[products:S... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:**\\ ... _2_species_with_Image_1.png }} * Highlight the file ''ATTO655+Cy5_diff_FCS+FLCS.ptu'' by a single mou... **Response:** The FLCS script is applied to the file ''ATTO655+Cy5_diff_FCS+FLCS.ptu''. Thereby, a new
using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs
14 Hits, Last modified:
===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[products:S... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:** Th... moval_via_flccs_Image_1.png }} * Highlight the file ''IBA488+IBA547_unlinked_mix.ptu'' by a single mouse click. **Note:** This file contains a FCS measurement of a FCCS negative con
calculate_fccs_trace_with_the_grouped_fcs_script
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===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[products:S... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:** Th... calculate_fccs_Image_1.png }} * Highlight the file ''IBA488+547_crosslinked.ptu'' by a single mouse click. **Note:** This file contains a FCS measurement of a FCCS positive con
visualizing_dynamics_using_the_multiframe-flim_script
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===== Step-by-Step Tutorial ===== ==== Select a file and start the analysis ==== * Start SymPhoTime... on 2.5). * Open the "Tutorials" workspace via **File -> Open Workspace** from the main menu.\\ **Resp... e left side of the main window. * Highlight the file ''Hyperosmotic_Shock_MDCK_Cells.ptu'' ​by a singl... ponse:​**\\ ​ The FLIM analysis is applied to the file ''Hyperosmotic_Shock_MDCK_Cells.ptu''. Thereby, a
calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script
12 Hits, Last modified:
===== Step-by-Step Tutorial ===== ==== Select a file and generate a reference FCS curve ==== * Star... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:** Th... ibration_script_Image_1.png }} * Highlight the file ''ATTO4888_485nm_pulsed.ptu'' by a single mouse c... } **Response:** The FCS script is applied to the file ''ATTO488_485nm_pulsed.ptu''. Thereby, a new wind
visualizing_dynamics_with_the_multi_frame_flim_analysis
12 Hits, Last modified:
===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[products:S... ] software. * Open the "Samples" workspace via "File -> Open Workspace" from the main menu. **Note:**... lizing_dynamics_Image_1.png }} * Highlight the file ''GUVs.ptu'' by a single mouse click. {{ visualiz... * The "Multi Frame FLIM" script is applied to the file ''GUs.ptu''. Thereby, a new Window opens: {{ visu
intensity_time_trace_analysis
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===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[products:S... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:** Th... me_trace_script_Image_1.png }} * Highlight the file ''Cy5_immo_Lifetime_Trace.ptu'' by a single mouse click. **Note:** This file contains a fluorescence time trace of an immobili
lifetime-fitting_using_the_flim_script
10 Hits, Last modified:
determination_of_the_focal_width_with_the_focal
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update
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calculate_and_fit_fcs_traces_with_the_fcs_script
8 Hits, Last modified:
lifetime_fitting_using_the_flim_analysis
8 Hits, Last modified:
using_the_antibunching_script
8 Hits, Last modified:
calculate_ratiometric_fret-images
7 Hits, Last modified:
flim-fret_calculation_for_single_exponential_donors
7 Hits, Last modified:
lifetime-fitting_using_the_rapid_reconvolution_algorithm
7 Hits, Last modified:
registering_new_scripts
5 Hits, Last modified:
how_to_work_with_the_instrument_response_function_irf
4 Hits, Last modified:
phasor_analysis
4 Hits, Last modified:
howto
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pattern_matching
3 Hits, Last modified:
measuring_quantum_yield
1 Hits, Last modified: