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symphotime_tips_and_tricks @howto
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In detail, this tutorial covers: * Different file-types used or generated by SymPhoTime64 * Image... tional options and tools otherwise hidden ===== File-Type Overview ===== ^Workspace: may contain these Filetypes ^ Content of the file ^ How to get such a file in the workspace ^ How to open/process^ |''.ptu''|Raw data in a unified TTTR-f
syntax @wiki
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is behavior can be enabled in the [[doku>config]] file. Hint: If DokuWiki is a link, then it's enabled. ... ?php /** * Customization of the english language file * Copy only the strings that needs to be modifie... ats. To mitigate the problem, you can upload your file in different formats for maximum browser compatib... filename as the video and be either a jpg or png file. In the example above a ''video.jpg'' file would
calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script @howto
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===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[products:... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:** Th... pie-fret_script_Image_1.png }} * Highlight the file ''Cy3+Cy5_diff_PIE-FRET.ptu'' by a single mouse c... Response:** The PIE FRET script is applied to the file ''Cy3+Cy5_diff_PIE-FRET.ptu'' and a new window op
lifetime_fitting_using_the_tcpsc_fitting_script @howto
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}} ===== Step-by-Step Tutorial ===== Select a file and start the script * Start [[products:SymPho... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:** Th... _fitting_script_Image_1.png }} * Highlight the file ''ATTO655_diff_FLCS-pattern.ptu'' by a single mou... nse:** The TCSPC Fitting script is applied to the file ''ATTO655_diff_FLCS-pattern.ptu''. Thereby, a new
calculate_ratiometric_single_pair_fret_distributions @howto
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===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[products:S... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:** Th... t_distributions_Image_1.png }} * Highlight the file ''Cy3+Cy5_diff_PIE-FRET.ptu'' by a single mouse c... **Response:** The FRET script is applied to the file ''Cy3+Cy5_diff_PIE-FRET.ptu''. Thereby, a new win
roi_fitting_using_the_flim_script @howto
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arge }} ===== Step-by-Step ===== ==== Select a file and start the script ==== * Start [[products:S... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:** Th... the_flim_script_Image_1.png }} * Highlight the file ''DaisyPollen_cells_FLIM.ptu'' by a single mouse ... **Response:** The FLIM script is applied to the file ''DaisyPollen_cells_FLIM.ptu''. Thereby, a new Wi
data_file_import @howto
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==== Data file import ==== Create a new workspace or open an existing one: {{ :howto:et2_createopen.png | File menu }} Then select Import Files. A new dialog opens, where the data file is selected: {{ :howto:et2_fileimport.png | Selects the file to be imported }} The file selected above is a s
separation_of_2_species_with @howto
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===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[products:S... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:**\\ ... _2_species_with_Image_1.png }} * Highlight the file ''ATTO655+Cy5_diff_FCS+FLCS.ptu'' by a single mou... **Response:** The FLCS script is applied to the file ''ATTO655+Cy5_diff_FCS+FLCS.ptu''. Thereby, a new
flim_fret_calculation_for_multi_exponential_donors @howto
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4 software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:**\\ ... onential_donors_Image_1.png }} * Highlight the file ''CENP-labelled _cells_for_FRET.ptu'' by a single... **Response:**\\ The FLIM script is applied to the file ''CENP-labelled_cells_for_FRET.ptu''. Thereby, a ... png }} **Response:**\\ A window opens, where the file containing the IRF can be selected. * Select th
using_the_anisotropy_image_script @howto
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===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[products:S... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:** Th... mage_script_Image_1.png?217 }} * Highlight the file ''Cy5_immo_FLIM+Pol-imaging''. {{ using_the_aniso... :** The Anisotropy Image script is applied to the file ''Cy5_immo_FLIM+Pol-Imaging.ptu''. Thereby, a new
using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs @howto
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===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[products:S... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:** Th... moval_via_flccs_Image_1.png }} * Highlight the file ''IBA488+IBA547_unlinked_mix.ptu'' by a single mouse click. **Note:** This file contains a FCS measurement of a FCCS negative con
calculate_fccs_trace_with_the_grouped_fcs_script @howto
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===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[products:S... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:** Th... calculate_fccs_Image_1.png }} * Highlight the file ''IBA488+547_crosslinked.ptu'' by a single mouse click. **Note:** This file contains a FCS measurement of a FCCS positive con
visualizing_dynamics_using_the_multiframe-flim_script @howto
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===== Step-by-Step Tutorial ===== ==== Select a file and start the analysis ==== * Start SymPhoTime... on 2.5). * Open the "Tutorials" workspace via **File -> Open Workspace** from the main menu.\\ **Resp... e left side of the main window. * Highlight the file ''Hyperosmotic_Shock_MDCK_Cells.ptu'' ​by a singl... ponse:​**\\ ​ The FLIM analysis is applied to the file ''Hyperosmotic_Shock_MDCK_Cells.ptu''. Thereby, a
visualizing_dynamics_with_the_multi_frame_flim_analysis @howto
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===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[products:S... ] software. * Open the "Samples" workspace via "File -> Open Workspace" from the main menu. **Note:**... lizing_dynamics_Image_1.png }} * Highlight the file ''GUVs.ptu'' by a single mouse click. {{ visualiz... * The "Multi Frame FLIM" script is applied to the file ''GUs.ptu''. Thereby, a new Window opens: {{ visu
intensity_time_trace_analysis @howto
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===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[products:S... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:** Th... me_trace_script_Image_1.png }} * Highlight the file ''Cy5_immo_Lifetime_Trace.ptu'' by a single mouse click. **Note:** This file contains a fluorescence time trace of an immobili
calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script @howto
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lifetime-fitting_using_the_flim_script @howto
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software
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determination_of_the_focal_width_with_the_focal @howto
9 Hits, Last modified:
update @howto
9 Hits, Last modified:
calculate_and_fit_fcs_traces_with_the_fcs_script @howto
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lifetime_fitting_using_the_flim_analysis @howto
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using_the_antibunching_script @howto
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lifetime-fitting_using_the_rapid_reconvolution_algorithm @howto
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calculate_ratiometric_fret-images @howto
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flim-fret_calculation_for_single_exponential_donors @howto
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registering_new_scripts @howto
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how_to_work_with_the_instrument_response_function_irf @howto
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howto @howto
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pre-histogrammed_image @glossary
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pattern_matching @howto
3 Hits, Last modified:
pycorrfit @software
3 Hits, Last modified:
create_time_gated_image
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phasor_analysis @howto
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supported_mt200_pc_configuration_for_symphotime_32 @software
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configuring_symphotime64_after_installation @support
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bdf @glossary
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measuring_quantum_yield @howto
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flimfit @software
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