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- lifetime-fitting_using_the_flim_script
- lly want to have in a pixel in order to perform a fit. If there are too few photons in a pixel, a decent fit result cannot be expected. It is also possible to... *Note:**\\ The software offers the possibility to fit the data using a n-exponential tailfit, n-exponential reconvolution or rapid reconvolution fit. A tailfit can be used when the fitted lifetimes are signifi... Response:**\\ A single exponential reconvolution fit is performed. In the TCSPC window, the fit is displayed as a black line. Below, the residual
- calculate_and_fit_fcs_traces_with_the_fcs_script
- is SymPhoTime}} ~~NOTOC~~ ====== Calculate and Fit FCS Traces with the FCS Script ====== ===== Summ... e left side of the main window. {{ calculate_and_fit_fcs_traces_with_the_fcs_script_Image_1.png }} ... .ptu'' by a single mouse click. {{ calculate_and_fit_fcs_traces_with_the_fcs_script_Image_2.png }} ... , open the dropdown menu "FCS". {{ calculate_and_fit_fcs_traces_with_the_fcs_script_Image_3.png }} **
- visualizing_dynamics_using_the_multiframe-flim_script
- *Note:**\\ The software offers the possibility to fit the data using a n-exponential tail fit, n-exponential reconvolution or rapid reconvolution fit. A tail fit can be used when the fitted lifetimes are signifi... Response:**\\ A single exponential reconvolution fit is performed. In the TCSPC window, the fit is displayed as a black line. Below, the residual... that the applied single exponential reconvolution fit is not suited to fit the decay curve. Increase the "Model Parameters"
- lifetime_fitting_using_the_flim_analysis
- *Note:**\\ The software offers the possibility to fit the data using a n-exponential tailfit or a n-exponential reconvolution fit. A tailfit can be used when the fitted lifetimes are signifi... nse:**\\ * A single exponential reconvolution fit is performed. In the TCSPC window, the fit is displayed as a black line. Below, the residual... that the applied single exponential reconvolution fit is not suited to fit the decay curve. * Increase the "Model Paramet... gain, click on "Initial fit". **Response:**\\ The fit quality increases, but still the fit quality is not sufficient. {{ lifetime-fitting_u
- roi_fitting_using_the_flim_script
- **Note:** The software offers the possibility to fit the data using a n-exponential tailfit or a n-exponential reconvolution fit. A tailfit can be used when the fitted lifetimes are signifi... . **Response: **\\ * In the TCSPC window, the fit of ROI 0 is displayed as a black line. Below, the residuals (= raw data fit values) are displayed. * Fit values appear in... that the applied single exponential reconvolution fit is not suited to fit the decay curve. * Increase the "Model Paramet... , click on "Initial fit": **Response:**\\ * The fit quality increases, but still the fit quality is not sufficient. {{ roi_fitting_using_t
- update
- *Note:**\\ The software offers the possibility to fit the data using an n-exponential tailfit, n-exponential reconvolution or rapid reconvolution fit. A tailfit can be used when the fitted lifetimes are signifi... Response:**\\ A single exponential reconvolution fit is performed. In the TCSPC window, the fit is displayed as a black line. Below, the residual... that the applied single exponential reconvolution fit is not suited to fit the decay curve. Increase the "Model Parameters"
- pattern_matching
- itting.\\ It is recommended to perform an initial fit before starting the Pattern Fit. This is because some parameters of the initial fit will be used in the Pattern Fit as start parameters and also because an initial fi... erns suffice to describe the image.\\ The initial fit {{initial_fit.png}} feature decomposes the overall decay (avera... n_matching_Image_16.png }} **Note:** The Pattern Fit {{pattern_fit.png}} algorithm is similar to the initial fitting
- flim_fret_calculation_for_multi_exponential_donors
- *Note:**\\ The software offers the possibility to fit the data using a n-exponential tailfit, a n-exponential reconvolution fit or a rapid reconvolution fit. A tailfit can be used when the fitted lifetimes are significantly longer than the i... strument response function. Still a reconvolution fit is usually preferable, especially for FRET determ... use the complete decay is fitted, while for a tailfit, the start of the fitting range is usually a bit
- lifetime-fitting_using_the_rapid_reconvolution_algorithm
- th ROI 0 and only one component (monoexponential) fit. Press "Initial Fit" (marked in orange). {{ howto:monoexpo_roi0.png |}} **Response:**\\ A single exponential fit is performed. In the TCSPC window, the fit is displayed as a black line. Below, the residuals (= difference between raw data and fit values) are displayed. Fit values appear in the fitting table. Check the fit... OI 0, fix the lifetimes by unchecking them in the fit table, as well as the IRF background and IRF Fit. * One the single pixel level, the background c
- lifetime_fitting_using_the_tcpsc_fitting_script
- offers the possibility to use a n-exponential tailfit or a n-exponential reconvolution model fit. A tailfit can be used when the expected lifetimes are signi... } **Response:** * In the TCSPC window, the fit is displayed as a black line. Below, the residuals (= raw data fit values) are displayed. * Fit values appear in... script_Image_16.png?600 }} * Click on "Initial Fit" to perform a monoexponential tailfit of the ATTO655 dataset. {{ lifetime_fitting_using... th dyes can be fitted with the same model, click "Fit All". **Response:** Both data sets are fitted wit
- separation_of_2_species_with
- cies_with_Image_16.png?600 }} * Press "Initial FIT" to fit these patterns to the decay of the FCS measuremen... urves can now be fitted by selecting "Transfer to fit". The process of how to fit a FCS curve is explained in the tutorial [[howto:... urves can now be fitted by selecting "Transfer to fit". The process of how to fit a FCS curve is explained in the tutorial [[howto:Calculate and Fit FCS Traces with the FCS Script]].
- reconvolution_fit
- d. The default fitting option is "Exponential Tailfit". In order to perform a reconvolution fit, we need to select and then assign a curve as an ... th IRF and decay curve time ranges. Pressing the "Fit Single" button performs a fully automated fit of a single exponential decay model: {{ :howto:e... s://www.picoquant.com/dl_manuals/discontinued/FluoFit_Manual.pdf|this manual]] of the predecessor of EasyTau2, the now discontinued FluoFit.
- visualizing_dynamics_with_the_multi_frame_flim_analysis
- e are many options in this panel, not all of them fit into the visible area of the screen. Thus, a scro... des the Fast Lifetime values. If a pixel-by-pixel fit has been performed, there are more arrays dependi... ing_dynamics_Image_19.png }} ==== Perform a FLIM Fit ==== **Note:** If not only the Fast FLIM image should be exported, but also a pixel-by-pixel fit for the whole stack is needed, a procedure accord
- flim-fret_calculation_for_single_exponential_donors
- ertain regions of the cytoplasm. A bi-exponential fit is performed in a larger region of interest to de... sence of [[glossary:fret|FRET]]. A pixel-by-pixel fit is performed in the image and in every pixel the ... 1expd_fittingmodel.png?400 |}} * Click "Initial Fit" below the fitting parameter. **Response:** The fit is performed to the ROI decay. {{ :howto:flim-fre
- symphotime_tips_and_tricks
- {{multiple_fits.png?400|}} * click on “initial fit” to get resonable staritng parameters, then “fit all” * When data are exported now, the fitting ... displays all fitting parameters side by side. {{fit-pannel_showall.png?200|}} {{fit-pannel_showallwindow.png?600|}} * Exporting fr