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- calculate_and_fit_fcs_traces_with_the_fcs_script
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- lifetime_fitting_using_the_tcpsc_fitting_script @howto
- mPhoTime}} ~~NOTOC~~ ====== SymPhoTime Lifetime Fitting ====== ===== Summary ===== This tutorial shows step-by-step, how to fit a selected model to a decay curve in order to get... As an example, a single exponential reconvolution fit is used to determine the lifetime of ATTO655 dilu... on the left side of the main window. {{ lifetime_fitting_using_the_tcpsc_fitting_script_Image_1.png }}
- roi_fitting_using_the_flim_script @howto
- ysis tutorial SymPhoTime}} ~~NOTOC~~ ====== ROI Fitting Using the FLIM Analysis ====== ===== Summar... ducts:SymPhoTime64|SymPhoTime 64]] can be used to fit several regions of interest (ROIs), and how to ex... regions of interest are defined which are finally fitted. {{ youtube>g6uyvLQW4nY?large }} ===== Step... panel on the left side of the main window. {{ roi_fitting_using_the_flim_script_Image_1.png }} * Hig
- lifetime_fitting_using_the_flim_analysis @howto
- utorial SymPhoTime}} ~~NOTOC~~ ====== Lifetime Fitting Using the FLIM Analysis ====== ===== Summar... f SymPhoTime 64 can be used to perform a lifetime fit and how to extract and read the results. In detai... pollen image in the "Samples" workspace, a suited fitting model is chosen and a pixel-by-pixel fit is performed. {{ youtube>bKzQ74_bvK4?large }} ===== Ste
- calculate_and_fit_fcs_traces_with_the_fcs_script @howto
- is SymPhoTime}} ~~NOTOC~~ ====== Calculate and Fit FCS Traces with the FCS Script ====== ===== Summ... fluorophore dissolved in water is calculated and fitted using the FCS script. {{ youtube>NRFaEuSb778?... e left side of the main window. {{ calculate_and_fit_fcs_traces_with_the_fcs_script_Image_1.png }} ... .ptu'' by a single mouse click. {{ calculate_and_fit_fcs_traces_with_the_fcs_script_Image_2.png }}
- lifetime-fitting_using_the_flim_script @howto
- mPhoTime MicroTime LSM_upgrade}} ====== Lifetime-Fitting Using the FLIM Analysis (updated for SymPhoTi... can be used to perform a pixel-by-pixel lifetime fit of a FLIM image and how to extract and read the r... //figshare.com/s/4957fcfa684daef86c23]], a suited fitting model is chosen and a pixel-by-pixel fit is performed. ===== Step-by-step Tutorial ===== ==== S
- update @howto
- analysis SymPhoTime MT LSM_upgrade}} ====== ROI Fitting Using the FLIM Analysis (updated for SymPhoTi... w the FLIM script of SymPhoTime 64 can be used to fit several regions of interest (ROIs), and how to ex... //figshare.com/s/4957fcfa684daef86c23]], a suited fitting model is chosen and a pixel-by-pixel fit is performed.\\ ===== Step-by-step Tutorial ===== ====
- visualizing_dynamics_using_the_multiframe-flim_script @howto
- t. The histogram is photon weighted.\\ **4: Decay Fitting Parameters**\\ Here the lifetime fitting model can be selected. Fitting is then applied to the lifetime graph(s) on the lower center ([[glo... nning_2.png |}} ==== Perform an Initial Lifetime Fit on the Selected Regions (ROIs) ==== * In the
- lifetime-fitting_using_the_rapid_reconvolution_algorithm @howto
- analysis SymPhoTime MT LSM_upgrade reconvolution fitting}} ====== Lifetime-Fitting Using the Rapid Reconvolution Model ====== ===== Introduction =====... p, how to use the Multi-Exponential Reconvolution fitting model with correction for rapidFLIM. This new approach allows better fitting for data acquired with rapidFLIM capable hard
- flim_fret_calculation_for_multi_exponential_donors @howto
- cted with the donor construct by performing a ROI-fit with the FLIM-analysis. The amplitude weighted av... y lifetime and also to perform the pixel-by-pixel fit to calculate the FRET efficiency. ===== Step-by-... the right of the plot.\\ **Lower left:** Lifetime fitting parameters. Here lifetime fitting models can be defined. Fitting is then applied to the lifetime g
- pattern_matching @howto
- ans, that probably two patterns are sufficient to fit the image. **Note:** Have a look at the //Decay ... ox. This allows for analyzing them with the TCSPC Fitting feature and calculating the values of the ave... and binding (not shown). ==== Perform a Pattern Fit ==== **Note:** The desired patterns are already ... and the non-FRET pattern) and can now be used for fitting.\\ It is recommended to perform an initial fi
- separation_of_2_species_with @howto
- tuations. Therefore, FCS curves of ATTO655 can be fitted with the simplest 3D diffusion model, making i... nce lifetime under certain conditions, a lifetime fit can be performed of the TCPSC histogram of the me... ment. The FLCS filters can be calculated from the fitted lifetime components. This approach called "LT (lifetime) fitting" can only be employed, if the two components
- symphotime_tips_and_tricks @howto
- meters are only available after performing a FLIM-Fit (i.e. the different lifetime components and ampli... refore have negative Fast Lifetime values. * in fitted images, constraints can be set (e.g. 0 as minimum lifetime) in the TCSPC fitting panel * the fast lifetime in an ideal syste... weighted average lifetime [τ av. int.]. Usually, fitted lifetimes tend to be slightly shorter, as the
- visualizing_dynamics_with_the_multi_frame_flim_analysis @howto
- e are many options in this panel, not all of them fit into the visible area of the screen. Thus, a scro... right of the plot.\\ - **Lower left:** Lifetime fitting parameters allowing to define the lifetime fitting models. Fitting is then applied to the lifetime graph(s) in the [[glossary:TCSPC]] histogram.\\
- reconvolution_fit @howto
- ===== Reconvolution fit ===== Select an ''.etc'' container that contains at least one IRF histogram and at least one decay curve. Open the Fitting Window by pressing {{:howto:ff.png?40|}}. {{... cay curve in this case, is displayed. The default fitting option is "Exponential Tailfit". In order to perform a reconvolution fit, we need to select and then assign a curve as an
- flim-fret_calculation_for_single_exponential_donors @howto
- ertain regions of the cytoplasm. A bi-exponential fit is performed in a larger region of interest to de... sence of [[glossary:fret|FRET]]. A pixel-by-pixel fit is performed in the image and in every pixel the ... e TCSPC histogram. On the left of this graph, the fitting model and parameter controls are displayed. ... from_thresh_2_v3.png?400 |}} * In the lifetime fitting panel, keep the default Fitting model "Mono-E