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- calculate_and_fit_fcs_traces_with_the_fcs_script
- lifetime_fitting_using_the_flim_analysis
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- reconvolution_fit (howto)
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Fulltext results:
- calculate_and_fit_fcs_traces_with_the_fcs_script @howto
- is SymPhoTime}} ~~NOTOC~~ ====== Calculate and Fit FCS Traces with the FCS Script ====== ===== Summ... e left side of the main window. {{ calculate_and_fit_fcs_traces_with_the_fcs_script_Image_1.png }} ... .ptu'' by a single mouse click. {{ calculate_and_fit_fcs_traces_with_the_fcs_script_Image_2.png }} ... , open the dropdown menu "FCS". {{ calculate_and_fit_fcs_traces_with_the_fcs_script_Image_3.png }} **
- visualizing_dynamics_using_the_multiframe-flim_script @howto
- *Note:**\\ The software offers the possibility to fit the data using a n-exponential tail fit, n-exponential reconvolution or rapid reconvolution fit. A tail fit can be used when the fitted lifetimes are signifi... Response:**\\ A single exponential reconvolution fit is performed. In the TCSPC window, the fit is displayed as a black line. Below, the residual... that the applied single exponential reconvolution fit is not suited to fit the decay curve. Increase the "Model Parameters"
- lifetime-fitting_using_the_flim_script @howto
- lly want to have in a pixel in order to perform a fit. If there are too few photons in a pixel, a decent fit result cannot be expected. It is also possible to... Response:**\\ A single exponential reconvolution fit is performed. In the TCSPC window, the fit is displayed as a black line. Below, the residual... that the applied single exponential reconvolution fit is not suited to fit the decay curve. Increase the "Model Parameters" ... s, IRF and Background. Then in the pixel-by-pixel fit, these values are kept constant. Do NOT click "Initial Fit" or "Fit" at this stage!\\ {{ :writingroom:3comp-exp-kidn
- lifetime_fitting_using_the_flim_analysis @howto
- *Note:**\\ The software offers the possibility to fit the data using a n-exponential tailfit or a n-exponential reconvolution fit. A tailfit can be used when the fitted lifetimes ... nse:**\\ * A single exponential reconvolution fit is performed. In the TCSPC window, the fit is displayed as a black line. Below, the residual... that the applied single exponential reconvolution fit is not suited to fit the decay curve. * Increase the "Model Paramet... gain, click on "Initial fit". **Response:**\\ The fit quality increases, but still the fit quality is not sufficient. {{ lifetime-fitting_u
- roi_fitting_using_the_flim_script @howto
- **Note:** The software offers the possibility to fit the data using a n-exponential tailfit or a n-exponential reconvolution fit. A tailfit can be used when the fitted lifetimes ... . **Response: **\\ * In the TCSPC window, the fit of ROI 0 is displayed as a black line. Below, the residuals (= raw data fit values) are displayed. * Fit values appear in... that the applied single exponential reconvolution fit is not suited to fit the decay curve. * Increase the "Model Paramet... , click on "Initial fit": **Response:**\\ * The fit quality increases, but still the fit quality is not sufficient. {{ roi_fitting_using_t
- update @howto
- Response:**\\ A single exponential reconvolution fit is performed. In the TCSPC window, the fit is displayed as a black line. Below, the residual... that the applied single exponential reconvolution fit is not suited to fit the decay curve. Increase the "Model Parameters" ... es fitted with the same model.\\ * Clicking on "Fit All" will fit all active data sets with the same model, using t... ers of the active data set. If you do not want to fit the complete decay (dataset 1) and the decay of a
- pattern_matching @howto
- itting.\\ It is recommended to perform an initial fit before starting the Pattern Fit. This is because some parameters of the initial fit will be used in the Pattern Fit as start parameters and also because an initial fi... erns suffice to describe the image.\\ The initial fit {{initial_fit.png}} feature decomposes the overall decay (avera... n_matching_Image_16.png }} **Note:** The Pattern Fit {{pattern_fit.png}} algorithm is similar to the initial fitting
- lifetime-fitting_using_the_rapid_reconvolution_algorithm @howto
- th ROI 0 and only one component (monoexponential) fit. Press "Initial Fit" (marked in orange). {{ howto:monoexpo_roi0.png |}} **Response:**\\ A single exponential fit is performed. In the TCSPC window, the fit is displayed as a black line. Below, the residuals (= difference between raw data and fit values) are displayed. Fit values appear in the fitting table. Check the fit... OI 0, fix the lifetimes by unchecking them in the fit table, as well as the IRF background and IRF Fit. * One the single pixel level, the background c
- flim_fret_calculation_for_multi_exponential_donors @howto
- *Note:**\\ The software offers the possibility to fit the data using a n-exponential tailfit, a n-exponential reconvolution fit or a rapid reconvolution fit. A tailfit can be used when the fitted lifetimes are significantly longer... strument response function. Still a reconvolution fit is usually preferable, especially for FRET determ... s, where the repetition rate has been too high to fit the decay completely into the TCSPC-window, e.g.
- separation_of_2_species_with @howto
- cies_with_Image_16.png?600 }} * Press "Initial FIT" to fit these patterns to the decay of the FCS measuremen... urves can now be fitted by selecting "Transfer to fit". The process of how to fit a FCS curve is explained in the tutorial [[howto:... urves can now be fitted by selecting "Transfer to fit". The process of how to fit a FCS curve is explained in the tutorial [[howto:Calculate and Fit FCS Traces with the FCS Script]].
- lifetime_fitting_using_the_tcpsc_fitting_script @howto
- } **Response:** * In the TCSPC window, the fit is displayed as a black line. Below, the residuals (= raw data fit values) are displayed. * Fit values appear in the fitting table. **Note:** Usually, a decent fit is characterized by the following criteria:\\ The... parameters is selected.\\ => In this example, the fit is already sufficient. * Store the result fil
- visualizing_dynamics_with_the_multi_frame_flim_analysis @howto
- e are many options in this panel, not all of them fit into the visible area of the screen. Thus, a scro... des the Fast Lifetime values. If a pixel-by-pixel fit has been performed, there are more arrays dependi... ing_dynamics_Image_19.png }} ==== Perform a FLIM Fit ==== **Note:** If not only the Fast FLIM image should be exported, but also a pixel-by-pixel fit for the whole stack is needed, a procedure accord
- flim-fret_calculation_for_single_exponential_donors @howto
- ertain regions of the cytoplasm. A bi-exponential fit is performed in a larger region of interest to de... sence of [[glossary:fret|FRET]]. A pixel-by-pixel fit is performed in the image and in every pixel the ... 1expd_fittingmodel.png?400 |}} * Click "Initial Fit" below the fitting parameter. **Response:** The fit is performed to the ROI decay. {{ :howto:flim-fre
- symphotime_tips_and_tricks @howto
- {{multiple_fits.png?400|}} * click on “initial fit” to get resonable staritng parameters, then “fit all” * When data are exported now, the fitting ... displays all fitting parameters side by side. {{fit-pannel_showall.png?200|}} {{fit-pannel_showallwindow.png?600|}} * Exporting fr
- reconvolution_fit @howto
- th IRF and decay curve time ranges. Pressing the "Fit Single" button performs a fully automated fit of a single exponential decay model: {{ :howto:e