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- calculate_and_fit_fcs_traces_with_the_fcs_script
- lifetime_fitting_using_the_flim_analysis
- lifetime_fitting_using_the_tcpsc_fitting_script
- lifetime-fitting_using_the_flim_script
- lifetime-fitting_using_the_rapid_reconvolution_algorithm
- reconvolution_fit (howto)
- roi_fitting_using_the_flim_script
- flimfit (software)
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- pycorrfit (software)
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- calculate_and_fit_fcs_traces_with_the_fcs_script @howto
- is SymPhoTime}} ~~NOTOC~~ ====== Calculate and Fit FCS Traces with the FCS Script ====== ===== Summ... e left side of the main window. {{ calculate_and_fit_fcs_traces_with_the_fcs_script_Image_1.png }} ... .ptu'' by a single mouse click. {{ calculate_and_fit_fcs_traces_with_the_fcs_script_Image_2.png }} ... , open the dropdown menu "FCS". {{ calculate_and_fit_fcs_traces_with_the_fcs_script_Image_3.png }} **
- visualizing_dynamics_using_the_multiframe-flim_script @howto
- nning_2.png |}} ==== Perform an Initial Lifetime Fit on the Selected Regions (ROIs) ==== * In the ... rop-down list select "n-Exponential Reconvolution Fit".\\ {{ howto:nexpon_fitmodel.png?300 |}} ** Res... *Note:**\\ The software offers the possibility to fit the data using a n-exponential tail fit, n-exponential reconvolution or rapid reconvolution fit. A tai
- lifetime-fitting_using_the_flim_script @howto
- can be used to perform a pixel-by-pixel lifetime fit of a FLIM image and how to extract and read the r... ited fitting model is chosen and a pixel-by-pixel fit is performed. ===== Step-by-step Tutorial ====... lly want to have in a pixel in order to perform a fit. If there are too few photons in a pixel, a decent fit result cannot be expected. It is also possible to
- lifetime_fitting_using_the_flim_analysis @howto
- f SymPhoTime 64 can be used to perform a lifetime fit and how to extract and read the results. In detai... ited fitting model is chosen and a pixel-by-pixel fit is performed. {{ youtube>bKzQ74_bvK4?large }} =... the fitting model to "n-Exponential Reconvolution Fit". {{ lifetime-fitting_using_the_flim-script_Image... *Note:**\\ The software offers the possibility to fit the data using a n-exponential tailfit or a n-exp
- roi_fitting_using_the_flim_script @howto
- ducts:SymPhoTime64|SymPhoTime 64]] can be used to fit several regions of interest (ROIs), and how to ex... ution of the raw image. ==== Perform a Lifetime Fit on the Selected Regions (ROIs) ==== * In the d... **Note:** The software offers the possibility to fit the data using a n-exponential tailfit or a n-exponential reconvolution fit. A tailfit can be used when the fitted lifetimes
- update @howto
- w the FLIM script of SymPhoTime 64 can be used to fit several regions of interest (ROIs), and how to ex... ited fitting model is chosen and a pixel-by-pixel fit is performed.\\ ===== Step-by-step Tutorial ===... orresponding to ''ROI 0'' will appear under decay fit in "Decay Fitting" panel.\\ **Note:**\\ For fin... n the list. ==== Perform an Initial Lifetime Fit on the Selected Regions (ROIs) ==== * In the
- pattern_matching @howto
- ans, that probably two patterns are sufficient to fit the image. **Note:** Have a look at the //Decay ... and binding (not shown). ==== Perform a Pattern Fit ==== **Note:** The desired patterns are already ... itting.\\ It is recommended to perform an initial fit before starting the Pattern Fit. This is because some parameters of the initial fit will be used in th
- lifetime-fitting_using_the_rapid_reconvolution_algorithm @howto
- is of SymPhoTime 64 is used to perform a lifetime fit and how to extract and read the results. A mixtur... dow and an extra gray decay on bottom right under Fit. Lifetime histogram and the preview FLIM image re... th ROI 0 and only one component (monoexponential) fit. Press "Initial Fit" (marked in orange). {{ howto:monoexpo_roi0.png |}} **Response:**\\ A single ex
- flim_fret_calculation_for_multi_exponential_donors @howto
- cted with the donor construct by performing a ROI-fit with the FLIM-analysis. The amplitude weighted av... y lifetime and also to perform the pixel-by-pixel fit to calculate the FRET efficiency. ===== Step-by-... *Note:**\\ The software offers the possibility to fit the data using a n-exponential tailfit, a n-exponential reconvolution fit or a rapid reconvolution fit. A tailfit can be us
- separation_of_2_species_with @howto
- nce lifetime under certain conditions, a lifetime fit can be performed of the TCPSC histogram of the me... cies_with_Image_16.png?600 }} * Press "Initial FIT" to fit these patterns to the decay of the FCS measurement. {{ separation_of_2_species_with_Image_17.p... 1 indicates that no further pattern is needed to fit the data. * Three FLCS filters are generated,
- lifetime_fitting_using_the_tcpsc_fitting_script @howto
- ===== This tutorial shows step-by-step, how to fit a selected model to a decay curve in order to get... As an example, a single exponential reconvolution fit is used to determine the lifetime of ATTO655 dilu... al tailfit or a n-exponential reconvolution model fit. A tailfit can be used when the expected lifetime... strument response function. Still a reconvolution fit is usually preferable, because the complete decay
- visualizing_dynamics_with_the_multi_frame_flim_analysis @howto
- e are many options in this panel, not all of them fit into the visible area of the screen. Thus, a scro... des the Fast Lifetime values. If a pixel-by-pixel fit has been performed, there are more arrays dependi... ing_dynamics_Image_19.png }} ==== Perform a FLIM Fit ==== **Note:** If not only the Fast FLIM image should be exported, but also a pixel-by-pixel fit for the whole stack is needed, a procedure accord
- flim-fret_calculation_for_single_exponential_donors @howto
- ertain regions of the cytoplasm. A bi-exponential fit is performed in a larger region of interest to de... sence of [[glossary:fret|FRET]]. A pixel-by-pixel fit is performed in the image and in every pixel the ... 1expd_fittingmodel.png?400 |}} * Click "Initial Fit" below the fitting parameter. **Response:** The fit is performed to the ROI decay. {{ :howto:flim-fre
- symphotime_tips_and_tricks @howto
- meters are only available after performing a FLIM-Fit (i.e. the different lifetime components and ampli... {{multiple_fits.png?400|}} * click on “initial fit” to get resonable staritng parameters, then “fit all” * When data are exported now, the fitting valu... displays all fitting parameters side by side. {{fit-pannel_showall.png?200|}} {{fit-pannel_showallwin
- software
- fitJS/ [[https://dwaithe.github.io/FCSfitJS/| FCS fit JS]] * [[software:FLIMFit]]. FLIMfit is an ope... * [[https://github.com/RobertMolenaar-UT/Lifetime_fit]] With this script you can fit TCSPC lifetime histograms to single, double or triple order exponential fit with reconvolution method Minimalization method a