This tutorial shows the recording of FLIM images using an LSM upgrade kit, in this case connected to an Olympus FluoView FV3000 laser scanning microscope.
General recording of FLIM images can also be found using the link below.
The general principles of the data acquisition are analogous in all LSM upgrade kits. The movie was recorded in 02/2018; the recording procedure may be slightly different in older or newer models.
Continue to: