FLIM Measurements Using an Olympus FV3000 with a PicoQuant FLIM Upgrade

video, FLIM, acquisition, howto, demo, Olympus, FV3000, LSM upgrade

This tutorial shows the recording of FLIM images using an LSM upgrade kit, in this case connected to an Olympus FluoView FV3000 laser scanning microscope.

General recording of FLIM images can also be found using the link below.

The general principles of the data acquisition are analogous in all LSM upgrade kits. The movie was recorded in 02/2018; the recording procedure may be slightly different in older or newer models.

Continue to:

How to Measure the Instrument Response Function (IRF)
How to Work with Instrument Response Functions (IRFs) Measured with a Microscope

FLIM-FRET Calculation for Multi Exponential Donors
FLIM-FRET Calculation for Single Exponential Donors
Lifetime Fitting Using the FLIM Analysis
Lifetime-Fitting Using the FLIM Analysis (updated for SymPhoTime V 2.5 and above)
Lifetime-Fitting Using the Rapid Reconvolution Model
Pattern Matching
Phasor Analysis
ROI Fitting Using the FLIM Analysis
ROI Fitting Using the FLIM Analysis (updated for SymPhoTime v2.5)
Visualizing Dynamics Using the Multi Frame FLIM Analysis
Visualizing Dynamics Using the Multi Frame FLIM Analysis (updated for SymPhoTime v2.5 and above)