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fcs
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====== Fluorescence Correlation Spectroscopy (FCS) ====== Fluorescence correlation spectroscopy (FCS) is a correlation analysis of fluctuation of the fluorescence intensity. The analysis provides parameters of th... (molecules) in solution. In this application, the fluorescence emitted from a very tiny space in solution contai
pile-up_effect
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ime for TCSPC devices used for the measurement of fluorescence decays are in the range of 25 to 90 ns. Let's t... an example a laser repetition rate of 40 MHz. The fluorescence molecules receive every 25 ns a laser pulse. Cons... stics: The probability of detecting more than one fluorescence photon per excitation pulse is negligible. * [
flim
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F**luorescence **LI**fetime **M**icroscopy. ([[wp>Fluorescence-lifetime imaging microscopy]]). In FLIM addition... lse color plot is used to visualize the average [[fluorescence lifetime]] in each pixel of the image. ===== FLI
fluorescence_lifetime
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====== Fluorescence Lifetime ====== [[wp>Fluorescence#Lifetime]]
convolution
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_{-\infty}^t F(t-t')~G(t')~dt'$ In time resolved fluorescence it describes the effects of an IRF with finite wi
mcs
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. What immediately results is a time trace of the fluorescence intensity. In a subsequent analysis the binned en
support_plane_analysis
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es ===== *Lakowicz JR (1999)// Principles of Fluorescence Spectroscopy//, 2nd edn. Kluver Academic/Plenum P
t2-mode
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two input channels. For all practically relevant fluorescence detection applications the effective rate per cha
t3-mode
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for input channel 1. For all practically relevant fluorescence detection applications this is more than sufficie
tcspc
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lapsed since the last excitation pulse. It is a time domain method for investigating time resolved fluorescence.