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- privacy_policy
- ezogene Daten Personenbezogene Daten sind alle Informationen, die sich auf eine identifizierte oder ident... nbezogenen Daten ohne Hinzuziehung zusätzlicher Informationen nicht mehr einer spezifischen betroffenen Pe... ordnet werden können, sofern diese zusätzlichen Informationen gesondert aufbewahrt werden und technischen ... zbar. **5. Erfassung von allgemeinen Daten und Informationen** Die Internetseite der PicoQuant GmbH erfa
- syntax @wiki
- ====== Formatting Syntax ====== [[doku>DokuWiki]] supports some simple markup language, which tries to ma... ku>toolbar|quickbuttons]], too. ===== Basic Text Formatting ===== DokuWiki supports **bold**, //italic//... wiki:dokuwiki-128.png}}]] Please note: The image formatting is the only formatting syntax accepted in link names. The whole [[#images_and_other_files|image]] an
- symphotime_tips_and_tricks @howto
- pen/process^ |''.ptu''|Raw data in a unified TTTR-format|<WRAP> - Generated by a measurement - Import ... ment file, contains manually entered text (e.g. information about the experiment)|Menu: **File → Create Co... ta) file opens a file viewer, in which you find information about the file type and acquisition settings <imgcaption image1| ''.ptu'' file information and comment > {{info_image.png?800| }} </imgca
- software
- please check if they use current versions of file formats and/or programming libraries before you invest i... rpose FCS evaluation software that, amongst other formats, supports the established Zeiss ConfoCor3 ~.fcs file format. PyCorrFit comes with several built-in model func... //github.com/PicoQuant/PicoQuant-Time-Tagged-File-Format-Demos| Demo Code for reading PicoQuants PTU File
- calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script @howto
- gle FRET pair can be analyzed, e.g. to observe conformational changes.\\ To record such traces, usually si... h was used to excite the Cy5 dye directly. This information is used to distinguish molecules with and with... lecules as in this example file. For observing conformational fluctuations of a single immobilized molecul... 1. The other two peaks are due to twodifferent conformations of the RNA oligonucleotide measured in this s
- pycorrfit @software
- rpose FCS evaluation software that, amongst other formats, supports the established Zeiss ConfoCor3 ~.fcs file format. PyCorrFit comes with several built-in model func... ]] PyCorrFit does support the PicoQuant t3r file format (currently only '''*.pt3''') [[https://github.com... ncement request for implementing '''*.ptu''' file format support is existing. [[https://github.com/paulmue
- calculate_ratiometric_single_pair_fret_distributions @howto
- gle FRET pair can be analyzed, e.g. to observe conformational changes.\\ To record such traces, usually si... h was used to excite the Cy5 dye directly. This information is not used in this script (in fact, for this ... lecules as in this example file. For observing conformational fluctuations of a single immobilized molecul... . The other two peaks are due to two different conformations of this RNA oligonucleotide measured in this
- fluorescence_correlation_spectroscopy-_a_short_introduction @general
- relation function ===== In general all relevant information is conveyed by the normalised autocorrelation ... or because of the internal flexibility of the conformation of the biomolecule the quencher comes closer t... luorescence one can infer the timescale of the conformational changes of the molecule under study. \\ {{:general:conformational_change_quencher.png?200|}} ==== A bit more
- calculate_and_fit_fcs_traces_with_the_fcs_script @howto
- youtube>NRFaEuSb778?large }} ===== Background Information ===== * ATTO488 (ATTO-Tec, Germany) is a su... odel and see the fitting parameter. If you need information about how the fitting models are defined and w... et al., 2005, Biophys. J. 89, 4029-4042)).\\ **Conformational:** Use this model if your dye is undergoing conformational changes when diffusing through the confocal
- visualizing_dynamics_using_the_multiframe-flim_script @howto
- rts the image as an ASCII array using a text-file format. Thereby one array encodes the intensity values, ... mage series as a series of binary files. The data format of these files is described on the SymPhoTime Online help. The binary files contain the photon information of each TCSPC-channel and can be imported e.g.... icroTime system, or you transfer the image size information automatically via a handshake software, as in
- imprint
- bute the materials on this website for personal information purposes only. Please note that any product, p... Quant GmbH logo are registered trademarks. This information could contain technical inaccuracies or typographical errors, and information will be changed, updated and deleted without n
- calculate_ratiometric_fret-images @howto
- youtube>rOas2iDwUzY?large }} ===== Background Information ===== [[glossary:fret|FRET]] is a method to determine molecular interactions or conformational changes based on a suited donor/acceptor pai... " and "7" for "Cross Talk". **Note:** For more information about the equation used for image calculation,
- pattern_matching @howto
- easurements. For more details, see **Additional Information** at the end of this step-by-step manual.\\ Pa... by adding a background pattern (see Additional Information at the end of this step-by-step manual). From ... rn_matching_Image_22.png?600 }} === Additional Information === **Note:** There is a lot of possibilities
- using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs @howto
- youtube>O2lTTF70tZE?large }} ===== Background Information ===== **Note:** Fluorescence Cross-Correlatio... elation that involves the fluorescence lifetime information. As the fluorescence lifetime information is used to remove a false positive contribution due to spect
- basics
- ques and instrumentation. \\ * Many useful information can be found in [[https://www.microscopyu.com/... * [[ https://www.microscopyu.com/tutorials/imageformation-airyna |Numerical Aperture and Image Resolutio