e achievable time resolution of the system is the FWHM (full width of half maximum) of an IRF-measuremen... ht click, activate `Data Reader`, and try to find FWHM and see how broad it is. ((This IRF has been meas... lter (> 500 nm).))
{{ howto:irf_linear_scale_fwhm.png?800 |}}
In this example channel 2 was selec
2N.A. Objective, 640nm excitation
*excitation FWHM = 350 nm (typical for 4x out-coupler!, the 10x ou... e diameter of the excitation spot is given as its FWHM. However the airy disc diameter is bigger and the... ust be used.
=== Single Focus ===
$2ln(2)=\frac{FWHM^2}{w_0^2}$
$w_0=0.849 \cdot FWHM$
The diameter of the excitation spot therefore is:
$ d_{exc}=2 \cd