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symphotime_tips_and_tricks
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ns This tutorial assumes SymPhoTime V2.0 ===== General UI Philosophy ===== * right clicking on graphs... ent > {{info_image.png?800| }} </imgcaption> The general file information contains for example: * the n... specially important for LSM upgrade kits where in general several frames are recorded, while for an image a... on the scaling of the color-encoded component (in general, the lifetime). Changing the color scale can also
how_to_measure_the_instrument_response_function_irf
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r to the fluorescence emission wavelength. ==== General recipe ==== * Create a saturated aqueous KI (p... ay. Our guess is that this quenching trick works generally. Specifically, we found that classic Fluorescei... the color dependence of SPAD detectors, which are generally used in microscopy. Furthermore, even with dete
flim-fret_measurement_using_an_olympus_fv1200_with_a_flim_and_fcs_upgrade
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principles are similar as for any FLIM-recording. General recording of FLIM images can also be found using the link below. The general principles of the data acquisition are analogous
data_file_import
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iner. This will make the data analysis easier. In general, such a ''.phu'' file may contain many more histo
flim_measurement_using_a_nikon_a1_with_a_flim_and_fcs_upgrade
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shown in a separate video (see link below). The general principles of the data acquisition are analogous
how_to_work_with_the_instrument_response_function_irf
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ow be used for the fitting. **Note:** * In general, an indicator of the achievable time resolution o
pattern_matching
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This is done in terms of a linear combination. In general, for i Patterns it finds the corresponding amplit
howto
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64/Demo_workspace_SPT64.zip|here]] (1.5GB). ==== General ==== {{topic>tutorial +analysis -FCS -FRET -FLIM
update
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r-encoded component. Changing the color scale (in general the lifetime) can change the sensitivity of this