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- flim_fret_calculation_for_multi_exponential_donors
- nter/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1). In red a... the right of the plot.\\ **Lower left:** Binding histogram. As the FRET efficiency image has not been calculated yet, this histogram is also still empty. As this diagram can only be ... istance histogram.\\ **Lower center/left:** TCSPC histogram of all photons in the image in green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1). In red a... **\\ Left to right: Preview FLIM Image, Intensity Histogram and Lifetime Histogram. For any ROI you can set each of these parameters
- calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script
- s still empty.\\ - **Right:** FRET distribution histogram and the FRET radius histogram. As these distributions have not been calculated ... ng ("PIE-FRET") are plotted. * Below the "E/S Histogram", the FRET efficiency histogram is plotted again including PIE filtering. * At the bottom, the distance histogram gives the distance of donor and acceptor dye in t... fashion, also the values can be red from the E/S-Histogram. The context menu also has options for exporting
- flim-fret_calculation_for_single_exponential_donors
- the right of the plot. - **Lower left: Binding histogram.** As the FRET efficiency image has not been calculated yet, this histogram is still empty. This diagram can only be calculat... al decay kinetics. - **Lower center/left: TCSPC histogram of all photons in the image.** In green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1). In red a... **\\ Left to right: Preview FLIM Image, Intensity Histogram and Lifetime Histogram. For any ROI you can set each of these parameters... stinct peaks. * On the lower right, the Binding histogram is plotted. The Binding histogram plots the amplitude fraction of the donor lifetim
- lifetime-fitting_using_the_rapid_reconvolution_algorithm
- **\\ Left to right: Preview FLIM Image, Intensity Histogram and Lifetime Histogram. For any ROI you can set each of these parameters... al bars on the sides of the intensity or lifetime histograms; click and drag the bars to set the threshold. ... ra gray decay on bottom right under Fit. Lifetime histogram and the preview FLIM image remains as it has been... roi0.png |}} **Response:**\\ The image and the histogram will get updated. The rainbow scale contains now
- lifetime-fitting_using_the_flim_script
- **\\ Left to right: Preview FLIM Image, Intensity Histogram and Lifetime Histogram. For any ROI you can set each of these parameters... al bars on the sides of the intensity or lifetime histograms; click and drag the bars to set the threshold.\\... eview image of ''ROI 0'', as well as its Lifetime Histogram will get updated. A corresponding decays with gra... ound photons.\\ * An updated lifetime frequency histogram on the upper right. The individual lifetime compo
- calculate_ratiometric_fret-images
- o 100% FRET efficiency. * The FRET efficiency histogram and the Distances histogram are shown. {{ calculate_ratiometric_fret-images_w... ral possibilities to continue: * To adapt the histogram plots, change the plot parameters on the right of the histogram to the desired values and click "Recalculate". To
- update
- ale can be adapted.\\ **3: Fluorescence Lifetime Histogram**\\ Here the frequency of photon counts correspon... using the controls on the right of the plot. The histogram is photon weighted.\\ **4: Decay Fitting Paramete... graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\ **5: [[glossary:TCSPC]] Decay window**\\ By default, this set shows the [[glossary:TCSPC]] histogram of all photons in the image in green (= dataset 0
- calculate_ratiometric_single_pair_fret_distributions
- .\\ - **Lower center/right:** FRET distribution histogram and FRET radius histogram. As these distributions have not been calculated ... s region is used to calculate the FRET efficiency histogram. The unmarked white area shows the fluorescence r... ted, which is used in the calculation of the FRET histogram later. These values are shown as green andred lin... lculated and the "FRET Efficiency" and "Distance" histograms are displayed in the lower part of the image. *
- lifetime_fitting_using_the_flim_analysis
- nter/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1). In red a... the limit bars in the color scale "Fast Lifetime" histogram or by considering the peaks of the "Lifetime Histogram". Entering fixed values has the advantage that di... und photons. * An updated lifetime frequency histogram on the upper right. The fixed individual lifetime
- visualizing_dynamics_with_the_multi_frame_flim_analysis
- the limit bars in the color scale "Fast Lifetime" histogram or by considering the peaks of the "Lifetime Histogram". Entering fixed values has the advantage that di
- roi_fitting_using_the_flim_script
- nter/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1). In red a... the limit bars in the color scale "Fast Lifetime" histogram or by considering the peaks of the "Lifetime Histogram". Entering fixed values has the advantage that di
- visualizing_dynamics_using_the_multiframe-flim_script
- ale can be adapted.\\ **3: Fluorescence Lifetime Histogram**\\ Here the frequency of photon counts correspon... using the controls on the right of the plot. The histogram is photon weighted.\\ **4: Decay Fitting Paramete... graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\ **5: [[glossary:TCSPC]] Decay window**\\ By default, this set shows the [[glossary:TCSPC]] histogram of all photons in the image in green (= dataset 0
- separation_of_2_species_with
- e above highlighted in green. The photon counting histogram on the right displays the frequencies of the diff... are available, it is possible to load these TCSPC histograms as patterns for the filter definition. This is c... Matching" approach. * If the individual TCSPC histograms are not available, or a dye is analyzed that cha... ons, a lifetime fit can be performed of the TCPSC histogram of the measurement. The FLCS filters can be calcu
- data_file_import
- \ Unlike ''Decay_Coumarin_6.phu'' containing two histograms, both ''Anthracene_EtOH.phu'' and ''IRF375.phu'' contains only one histogram. These two measurements actually belong to each o
- lifetime_fitting_using_the_tcpsc_fitting_script
- The TCSPC fitting window is opened and the TCSPC histograms of both files are loaded. The TCSPC histogram from the file "ATTO655_diff_FLCS-pattern.ptu is m... measurement of the dye Cy5 in water. In the TCSPC histogram it can be clearly seen that the lifetime of this