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- flim_fret_calculation_for_multi_exponential_donors
- nter/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1). In red a... the right of the plot.\\ **Lower left:** Binding histogram. As the FRET efficiency image has not been calculated yet, this histogram is also still empty. As this diagram can only be ... istance histogram.\\ **Lower center/left:** TCSPC histogram of all photons in the image in green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1). In red a... **\\ Left to right: Preview FLIM Image, Intensity Histogram and Lifetime Histogram. For any ROI you can set each of these parameters
- flim-fret_calculation_for_single_exponential_donors
- the right of the plot. - **Lower left: Binding histogram.** As the FRET efficiency image has not been calculated yet, this histogram is still empty. This diagram can only be calculat... al decay kinetics. - **Lower center/left: TCSPC histogram of all photons in the image.** In green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1). In red a... **\\ Left to right: Preview FLIM Image, Intensity Histogram and Lifetime Histogram. For any ROI you can set each of these parameters... stinct peaks. * On the lower right, the Binding histogram is plotted. The Binding histogram plots the amplitude fraction of the donor lifetim
- calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script
- s still empty.\\ - **Right:** FRET distribution histogram and the FRET radius histogram. As these distributions have not been calculated ... ng ("PIE-FRET") are plotted. * Below the "E/S Histogram", the FRET efficiency histogram is plotted again including PIE filtering. * At the bottom, the distance histogram gives the distance of donor and acceptor dye in t... fashion, also the values can be red from the E/S-Histogram. The context menu also has options for exporting
- lifetime-fitting_using_the_rapid_reconvolution_algorithm
- **\\ Left to right: Preview FLIM Image, Intensity Histogram and Lifetime Histogram. For any ROI you can set each of these parameters... ra gray decay on bottom right under Fit. Lifetime histogram and the preview FLIM image remains as it has been... roi0.png |}} **Response:**\\ The image and the histogram will get updated. The rainbow scale contains now ... time", based on the photon arrival time only. The Histogram on the right of the image contains now the photon
- lifetime-fitting_using_the_flim_script
- **\\ Left to right: Preview FLIM Image, Intensity Histogram and Lifetime Histogram. For any ROI you can set each of these parameters... eview image of ''ROI 0'', as well as its Lifetime Histogram will get updated. A corresponding decays with gra... ound photons.\\ * An updated lifetime frequency histogram on the upper right. The individual lifetime compo
- update
- ale can be adapted.\\ **3: Fluorescence Lifetime Histogram**\\ Here the frequency of photon counts correspon... using the controls on the right of the plot. The histogram is photon weighted.\\ **4: Decay Fitting Paramete... graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\ **5: [[glossary:TCSPC]] Decay window**\\ By default, this set shows the [[glossary:TCSPC]] histogram of all photons in the image in green (= dataset 0
- lifetime_fitting_using_the_flim_analysis
- nter/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1). In red a... the limit bars in the color scale "Fast Lifetime" histogram or by considering the peaks of the "Lifetime Histogram". Entering fixed values has the advantage that di... und photons. * An updated lifetime frequency histogram on the upper right. The fixed individual lifetime
- visualizing_dynamics_with_the_multi_frame_flim_analysis
- the limit bars in the color scale "Fast Lifetime" histogram or by considering the peaks of the "Lifetime Histogram". Entering fixed values has the advantage that di
- roi_fitting_using_the_flim_script
- nter/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1). In red a... the limit bars in the color scale "Fast Lifetime" histogram or by considering the peaks of the "Lifetime Histogram". Entering fixed values has the advantage that di
- visualizing_dynamics_using_the_multiframe-flim_script
- ale can be adapted.\\ **3: Fluorescence Lifetime Histogram**\\ Here the frequency of photon counts correspon... using the controls on the right of the plot. The histogram is photon weighted.\\ **4: Decay Fitting Paramete... graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\ **5: [[glossary:TCSPC]] Decay window**\\ By default, this set shows the [[glossary:TCSPC]] histogram of all photons in the image in green (= dataset 0
- calculate_ratiometric_fret-images
- o 100% FRET efficiency. * The FRET efficiency histogram and the Distances histogram are shown. {{ calculate_ratiometric_fret-images_w... ral possibilities to continue: * To adapt the histogram plots, change the plot parameters on the right of the histogram to the desired values and click "Recalculate". To
- calculate_ratiometric_single_pair_fret_distributions
- .\\ - **Lower center/right:** FRET distribution histogram and FRET radius histogram. As these distributions have not been calculated ... s region is used to calculate the FRET efficiency histogram. The unmarked white area shows the fluorescence r... ted, which is used in the calculation of the FRET histogram later. These values are shown as green andred lin... otide measured in this sample. * The distance histogram gives the distance of donor and acceptor dye in t
- separation_of_2_species_with
- e above highlighted in green. The photon counting histogram on the right displays the frequencies of the diff... ons, a lifetime fit can be performed of the TCPSC histogram of the measurement. The FLCS filters can be calcu... different parameters. - **Upper center:** TCSPC histogram of the imported patterns. As we haven't loaded an... mage_11.png?384 }} **Response:** * The TCSPC histogram for this file appears in the "Patterns" graph in
- using_the_anisotropy_image_script
- olecules to calculate the static anisotropy and a histogram with the anisotropy distribution. {{ youtube>Tyc... w opens which contains the anisotropy image and a histogram with the anisotropy distribution over the differe... tween -0.4 and 1. - **Lower graph:** Anisotropy histogram over the different pixels. **Note:** The anisot... the display settings for the anisotropy frequency histogram in the corresponding controls. {{ using_the_aniso
- pattern_matching
- the picture. The //Decay Diversity Map// is a 2D histogram. The x-axis shows a histogram of the average lifetimes of the pixels in the image. The y-axis shows a histogram of the Delta-Tau values of each pixel in the imag