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--- howto:calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script [2014/02/13 13:29] – external edit 127.0.0.1
+++ howto:calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script [2014/06/18 09:33] – admin
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-====== Calibrate the Confocal Volume using the FCS-Calibration Analysis ======
+{{tag> tutorial howto analysis calibration PSF confocal-volume VEFF FCS SymPhoTime}}
+~~NOTOC~~
+====== Calibrate the Confocal Volume for and with FCS ======
 ===== Summary =====
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 This tutorial shows step-by-step, how to calibrate the confocal volume for FCS measurements with a calibration dye. Therefore, a FCS curve is calculated with the FCS script, that is transferred to the FCS Calibration script.
+{{ youtube>_dyVx-l_tY?large }}
 ===== Background Information =====
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 {{ calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script_Image_7.png }}
-                                                  ⇓
+;#;
+⇓
+;#;
 {{ calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script_Image_8.png }}
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   * Click on "Yes" to store the data, click on "No" if you want to keep the old user profile.