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--- howto:using_the_intensity_time_trace_script [2014/04/09 20:03] – admin
+++ howto:intensity_time_trace_analysis [2014/06/19 08:12] – admin
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-{{tag> tutorial howto time-trace analysis}}
+{{tag> tutorial howto time-trace analysis SymPhoTime}}
 ~~TOC~~
-====== Using the Intensity Time Trace Analysis ======
+======Intensity Time Trace Analysis======
@@ Line 10: / Line 10: @@
 This tutorial shows step-by-step, how to draw intensity time traces from a single molecule. The script requires a point measurement of an immobilized fluorophore. When applied to images, it can be used to check for line and frame marker presence and assignment.
+{{ youtube>D4BBTLBvanQ?large }}
  \\
@@ Line 19: / Line 22: @@
   * Open the "Samples" workspace via "File\open Workspace" from the main menu.
-**Note:** The "Samples" workspace is delivered with the SymPhoTime 64 and on the CD-ROM and contains example data to show the function of the SymPhoTime data analysis. If you haven't installed it on your computer, copy it from the DVD onto a local drive before going through this tutorial, e.g. to a folder "C:\SPT_User".
+**Note:** The "Samples" workspace is delivered with the SymPhoTime 64 and on the CD-ROM and contains example data to show the function of the SymPhoTime data analysis. If you haven't installed it on your computer, copy it from the DVD onto a local drive before going through this tutorial, e.g. to a folder ''C:\SPT_User''.
 **Response:** The files of the sample workspace are displayed in the workspace panel on the left side of the main window.
@@ Line 100: / Line 103: @@
   * You can zoom out again by activating the context menu with a right mouse click and select "Rescale".
   * In this way, the marker assignment for this file has been checked and the window can be closed. As we are not interested in the data itself, there is no need to store this file.