howto:lifetime-fitting_using_the_flim_script
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howto:lifetime-fitting_using_the_flim_script [2020/05/12 14:27] – ↷ Page moved from writingroom:lifetime-fitting_using_the_flim_script to howto:lifetime-fitting_using_the_flim_script ruckelshausen | howto:lifetime-fitting_using_the_flim_script [2020/05/12 14:35] – ↷ Links adapted because of a move operation ruckelshausen | ||
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The file '' | The file '' | ||
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* Open the " | * Open the " | ||
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A large window with three sections will appear, where you can set a threshold for an image analysis visually. | A large window with three sections will appear, where you can set a threshold for an image analysis visually. | ||
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* In the fourth box, "Decay Fitting", | * In the fourth box, "Decay Fitting", | ||
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* From " | * From " | ||
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* In this example it is obvious that the applied single exponential reconvolution fit is not suited to fit the decay curve. Increase the "Model Parameters" | * In this example it is obvious that the applied single exponential reconvolution fit is not suited to fit the decay curve. Increase the "Model Parameters" | ||
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* Three components exponential decay seems to be suitable for this ROI. However we can try four exponential decay and see the result:\\ | * Three components exponential decay seems to be suitable for this ROI. However we can try four exponential decay and see the result:\\ | ||
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* Set the same limit for A2 and A3.\\ | * Set the same limit for A2 and A3.\\ | ||
* Uncheck the boxes for the individual lifetime components, Bkgr(Decay), | * Uncheck the boxes for the individual lifetime components, Bkgr(Decay), | ||
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* Click "FLIM Fit" in the upper panel.\\ | * Click "FLIM Fit" in the upper panel.\\ | ||
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* An updated lifetime frequency histogram on the upper right. The individual lifetime components are displayed as vertical bars, as they are not varied in the pixel-by-pixel fit.\\ | * An updated lifetime frequency histogram on the upper right. The individual lifetime components are displayed as vertical bars, as they are not varied in the pixel-by-pixel fit.\\ | ||
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* The three lifetime components can be displayed in a false color scale. Therefore switch the display to " | * The three lifetime components can be displayed in a false color scale. Therefore switch the display to " | ||
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**Note:**\\ | **Note:**\\ |
howto/lifetime-fitting_using_the_flim_script.txt · Last modified: 2023/08/03 14:16 by lan