Differences

This shows you the differences between two versions of the page.

--- writingroom:visualizing_dynamics_using_the_multiframe-flim_script [2020/04/29 03:18] – [Define image stack options and select ROI] rezvani
+++ writingroom:visualizing_dynamics_using_the_multiframe-flim_script [2020/05/12 13:08] – ↷ Links adapted because of a move operation ruckelshausen
@@ Line 16: / Line 16: @@
 **Response:**\\
 The files of the "Tutorials" workspace are displayed in the workspace panel on the left side of the main window.
-  * Highlight the file ''MDCK_Cells_FLIM.ptu'' by a single mouse click.
+  * Highlight the file ''Hyperosmotic_Shock_MDCK_Cells.ptu'' by a single mouse click.
   * Select the **"Analysis"** tab and in there, open the **"Imaging"** drop-down menu.\\
   * Start the "multi Frame FLIM" analysis by clicking on "Start".\\
@@ Line 23: / Line 23: @@
 **Response:**\\
-The FLIM analysis is applied to the file ''MDCK_Cells_FLIM.ptu''. Thereby, a new Window opens:
+The FLIM analysis is applied to the file ''Hyperosmotic_Shock_MDCK_Cells.ptu''. Thereby, a new Window opens:
 {{ :writingroom:fullscreen_tensionsensor_2.png |}}
@@ Line 99: / Line 99: @@
   * In the "Decay Fitting" panel, from the Fitting Model drop-down list select "n-Exponential Reconvolution Fit".\\
-{{ :writingroom:nexpon_fitmodel.png?400 |}}
+{{ :writingroom:nexpon_fitmodel.png?300 |}}
 ** Response:**\\
@@ Line 113: / Line 113: @@
   * From "Decay" drop-down list select ''ROI 0'' and click "Initial Fit" (marked in orange).\\
-<wrap hi>hier fehlt ein Screenshot</wrap>
+{{ :writingroom:selectroi_initfit_multiframe_2.png?300 |}}
 ** Response:**\\
 A single exponential reconvolution fit is performed. In the TCSPC window, the fit is displayed as a black line. Below, the residuals (= difference between raw data and fit values) are displayed.\\
   * Fit values appear in the fitting table. Check the fitting curve, residuals and χ².\\
+{{ :writingroom:monoexpo_roi0_10framebinning_2.png |}}
-<wrap hi>hier fehlt ein screenshot</wrap>
 **Note:**\\
@@ Line 132: / Line 131: @@
   * In this example it is obvious that the applied single exponential reconvolution fit is not suited to fit the decay curve. Increase the "Model Parameters" to n = 2. Again, click on "Initial fit".\\
-<wrap hi>hier fehlt noch ein Screenshot</wrap>
+{{ :writingroom:biexpo_roi0_10framebinning_2.png |}}
 ** Response:**\\
@@ Line 165: / Line 164: @@
 The average lifetime, you can see that after adding the sugar, has been dropped gradually about 500ps.\\
-  * If an IRF has been measured, the fits can also be performed using the measured IRFs. You need to import your measured IRF for the respective channel. Then repeat the above steps to find a decent fit. For more details please see <wrap hi> [[writingroom:how_to_work_with_the_instrument_response_function_irf|How to Work with Instrument Response Functions (IRFs) Measured with a Microscope]] hier muss der finale Link rein, nicht der Writingroom-Link</wrap> .  \\
+  * If an IRF has been measured, the fits can also be performed using the measured IRFs. You need to import your measured IRF for the respective channel. Then repeat the above steps to find a decent fit. For more details please see <wrap hi> [[howto:how_to_work_with_the_instrument_response_function_irf|How to Work with Instrument Response Functions (IRFs) Measured with a Microscope]] hier muss der finale Link rein, nicht der Writingroom-Link</wrap> .  \\