User Tools

Site Tools


howto:visualizing_dynamics_using_the_multiframe-flim_script

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

Both sides previous revisionPrevious revision
Next revision
Previous revision
Next revisionBoth sides next revision
writingroom:visualizing_dynamics_using_the_multiframe-flim_script [2020/04/29 03:42] – [Perform an Initial Lifetime Fit on the Selected Regions (ROIs)] rezvaniwritingroom:visualizing_dynamics_using_the_multiframe-flim_script [2020/04/30 09:36] – [Select a file and start the analysis] rezvani
Line 16: Line 16:
 **Response:​**\\ ​ **Response:​**\\ ​
 The files of the "Tutorials" workspace are displayed in the workspace panel on the left side of the main window. The files of the "Tutorials" workspace are displayed in the workspace panel on the left side of the main window.
-  * Highlight the file ''MDCK_Cells_FLIM.ptu'' ​by a single mouse click.+  * Highlight the file ''Hyperosmotic_Shock_MDCK_Cells.ptu'' ​by a single mouse click.
   * Select the **"​Analysis"​** tab and in there, open the **"​Imaging"​** drop-down menu.\\   * Select the **"​Analysis"​** tab and in there, open the **"​Imaging"​** drop-down menu.\\
   * Start the "multi Frame FLIM" analysis by clicking on "Start".\\   * Start the "multi Frame FLIM" analysis by clicking on "Start".\\
Line 23: Line 23:
  
 **Response:​**\\ ​ **Response:​**\\ ​
-The FLIM analysis is applied to the file ''MDCK_Cells_FLIM.ptu''. Thereby, a new Window opens:+The FLIM analysis is applied to the file ''Hyperosmotic_Shock_MDCK_Cells.ptu''. Thereby, a new Window opens:
  
 {{ :writingroom:fullscreen_tensionsensor_2.png |}} {{ :writingroom:fullscreen_tensionsensor_2.png |}}
Line 99: Line 99:
   * In the "Decay Fitting" panel, from the Fitting Model drop-down list select "n-Exponential Reconvolution Fit".\\   * In the "Decay Fitting" panel, from the Fitting Model drop-down list select "n-Exponential Reconvolution Fit".\\
  
-{{ :writingroom:nexpon_fitmodel.png?400 |}}+{{ :writingroom:nexpon_fitmodel.png?300 |}}
  
 ** Response:**\\ ** Response:**\\
Line 113: Line 113:
   * From "Decay" drop-down list select ''ROI 0'' and click "Initial Fit" (marked in orange).\\   * From "Decay" drop-down list select ''ROI 0'' and click "Initial Fit" (marked in orange).\\
  
-{{ :writingroom:selectroi_initfit_multiframe.png?400 |}}+{{ :writingroom:selectroi_initfit_multiframe_2.png?300 |}}
    
 ** Response:**\\  ** Response:**\\ 
howto/visualizing_dynamics_using_the_multiframe-flim_script.txt · Last modified: 2023/02/17 12:41 by admin