howto:visualizing_dynamics_using_the_multiframe-flim_script
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writingroom:visualizing_dynamics_using_the_multiframe-flim_script [2020/04/29 03:52] – [Perform an Initial Lifetime Fit on the Selected Regions (ROIs)] rezvani | writingroom:visualizing_dynamics_using_the_multiframe-flim_script [2020/05/12 15:51] – ruckelshausen | ||
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- | {{tag> | + | {{tag> |
====== Visualizing Dynamics using the Multi Frame FLIM Analysis ====== | ====== Visualizing Dynamics using the Multi Frame FLIM Analysis ====== | ||
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**Response: | **Response: | ||
The files of the " | The files of the " | ||
- | * Highlight the file '' | + | * Highlight the file '' |
* Select the **" | * Select the **" | ||
* Start the "multi Frame FLIM" analysis by clicking on " | * Start the "multi Frame FLIM" analysis by clicking on " | ||
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**Response: | **Response: | ||
- | The FLIM analysis is applied to the file '' | + | The FLIM analysis is applied to the file '' |
{{ : | {{ : | ||
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* In the "Decay Fitting" | * In the "Decay Fitting" | ||
- | {{ : | + | {{ howto: |
** Response: | ** Response: | ||
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The average lifetime, you can see that after adding the sugar, has been dropped gradually about 500ps.\\ | The average lifetime, you can see that after adding the sugar, has been dropped gradually about 500ps.\\ | ||
- | * If an IRF has been measured, the fits can also be performed using the measured IRFs. You need to import your measured IRF for the respective channel. Then repeat the above steps to find a decent fit. For more details please see <wrap hi> [[writingroom: | + | * If an IRF has been measured, the fits can also be performed using the measured IRFs. You need to import your measured IRF for the respective channel. Then repeat the above steps to find a decent fit. For more details please see <wrap hi> [[howto: |
howto/visualizing_dynamics_using_the_multiframe-flim_script.txt · Last modified: 2023/02/17 12:41 by admin