{{tag>tutorial howto analysis FLIM imaging FRET SymPhoTime}}
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====== FLIM-FRET Calculation for ... lysis" tab and in there, open the drop down menu "Imaging".
{{ flim-fret-calculation_for_multi-exponential_... tains five different regions:\\
**Upper left:** Imaging analysis options.\\
**Upper center:** Fast FLIM ... lysis" tab and in there, open the drop down menu "Imaging".
{{ flim-fret-calculation_for_multi-exponential_
e **"Analysis"** tab and in there, open the **"Imaging"** drop-down menu.\\
**Note:**\\
The drop-do... five different sections:\\
**1: Setting panel**\\
Imaging analysis options can be defined in this section.\
{{tag> howto FLIM imaging ROI analysis tutorial SymPhoTime}}
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====== ROI Fitting Using the FLIM ... lysis"-tab and in there, open the drop down menu "Imaging".
{{ roi_fitting_using_the_flim_script_Image_3.pn... ins five different regions:\\
- **Upper left:** imaging analysis options.\\
- **Upper center:** [[gloss
e **"Analysis"** tab and in there, open the **"Imaging"** drop-down menu.\\
* Start the FLIM script b... five different sections:\\
**1: Setting panel**\\
Imaging analysis options can be defined in this section.\
{{tag>howto FLIM multi-frame imaging analysis dynamics tutorial LSM_upgrade SymPhoTime}}
====== Visualizin... e **"Analysis"** tab and in there, open the **"Imaging"** drop-down menu.\\
* Start the "multi Frame ... five different sections:\\
**1: Setting panel**\\
Imaging analysis options can be defined in this section.\
the **"Analysis"** tab and in there, open the **"Imaging"** drop-down menu.
**Note:**\\
The drop-down me... five different sections:\\
**1: Setting panel**\\
Imaging analysis options can be defined in this section.\
{{tag> howto FLIM multi-frame imaging analysis dynamics tutorial SymPhoTime}}
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====== Visualizin... lysis" tab and in there, open the drop down menu "Imaging".
{{ visualizing_dynamics_Image_3.png?322 }}
**N... ins five different regions:\\
- **Upper left:** Imaging analysis options. As there are many options in th... not all GUVs are completely stationary during the imaging process indicated by blurring of certain GUVs.
=
{{tag>tutorial howto FRET FLIM imaging analysis SymPhoTime}}
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====== FLIM-FRET Calculation for ... 0/appnote_flim_overview.pdf|Fluorescence Lifetime Imaging (FLIM) in Confocal Microscopy Applications: An Ov... lysis" tab and in there, open the drop down menu "Imaging".
{{ :howto:flim-fret-1expd_analysis_imaging.png?500 |}}
**Note:** The drop down menu can be opened and c
tab or automatically for remotely controlled FLIM-imaging
* the imaging mode (mono- or bidirectional)
* whether it's a t2 or t3 type file
* Info that was ent...
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erision oil, without any coverslips etc.).
==== Imaging/ finding nv centers ====
*focus on the inte... x mismatch between oil and diamond)
*when imaging the surface most likely fluorescent spots will be
17 }}
* Highlight the file ''Cy5_immo_FLIM+Pol-imaging''.
{{ using_the_anisotropy_image_script_Image_2.p... lysis" tab and in there, open the drop down menu "Imaging".
{{ using_the_anisotropy_image_script_Image_3.pn... script is applied to the file ''Cy5_immo_FLIM+Pol-Imaging.ptu''. Thereby, a new window opens which contains... ow contains four different regions:
- **Left:** Imaging analysis and processing options.
- **Upper cent
{{tag> tutorial howto imaging FRET analysis SymPhoTime}}
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====== Calculate Ratiometric FRET Imag... lysis" tab and in there, open the drop down menu "Imaging".
{{ calculate_ratiometric_fret-images_with_the_f... ins five different regions:\\
- **Upper left:** imaging calculation analysis options.\\
- **Upper cente
{{tag>howto analysis FLIM imaging tutorial SymPhoTime}}
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====== Lifetime Fitting Using the FLIM... lysis" tab and in there, open the drop down menu "Imaging".
{{ lifetime-fitting_using_the_flim-script_Image... ontains five different regions:\\
**Upper left:** imaging analysis options.\\
**Upper center:** Fast FLIM i