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- how_to_measure_the_instrument_response_function_irf
- {{tag>howto IRF acquisition video Nikon LSM_upgrade}} ~~TOC~~ ====== How to Measure the Instrument Response Function (IRF) ====== (For an explanation of the term Instrument Response Function see [[glossary:IRF]]) The following video shows how to measure na IRF on confocal microscopes. {{youtube>cjSgCBe3ReM?la
- how_to_work_with_the_instrument_response_function_irf
- {{tag>howto IRF tutorial analysis MicroTime LSM_upgrade}} ====== How to Work with Instrument Response Functions (IRFs) Measured with a Microscope ====== ===== Introduction ===== IRF stands for Instrument Response Function, and it r... rument contribute to both shape and width of the IRF. This width is an indicator the timing resolutio
- flim_fret_calculation_for_multi_exponential_donors
- estimation of the instrument response function (=IRF) is shown. The IRF reconstruction is deducted from the rising edge of the TCSPC histogram. * As dat... } **Response:**\\ * In the TCSPC window, the IRF is displayed in bright red, the data fitting limi... C window. * The new fitting parameters "Shift IRF" and "Bkgr IRF" (=background IRF) appear in the f
- lifetime-fitting_using_the_flim_script
- estimation of the instrument response function (=IRF) is shown. The IRF reconstruction is deducted from the rising edge of the [[glossary:TCSPC]] histogram... 00 |}} ** Response:**\\ In the TCSPC window, the IRF is displayed in bright red, the data fitting limi... TCSPC window.\\ The new fitting parameters "Shift IRF" and "Bkgr IRF" (=background IRF) appear in the f
- visualizing_dynamics_using_the_multiframe-flim_script
- estimation of the instrument response function (=IRF) is shown. The IRF reconstruction is deducted from the rising edge of the [[glossary:TCSPC]] histogram... 00 |}} ** Response:**\\ In the TCSPC window, the IRF is displayed in bright red, the data fitting limi... TCSPC window.\\ The new fitting parameters "Shift IRF" and "Bkgr IRF" (=background IRF) appear in the f
- lifetime_fitting_using_the_flim_analysis
- estimation of the instrument response function (=IRF) is shown. The IRF reconstruction is deducted from the rising edge of the TCSPC histogram. * To enh... } **Response:**\\ * In the TCSPC window, the IRF is displayed in bright red, the data fitting limi... C window. * The new fitting parameters "Shift IRF" and "Bkgr IRF" (=background IRF) appear in the f
- lifetime_fitting_using_the_tcpsc_fitting_script
- del. **Response:** * In the TCSPC window, the IRF is displayed in bright red and the data fitting l... C window. * The new fitting parameters "Shift IRF" and "Bkgr IRF" (=background IRF) appear in the fitting parameter table. {{ lifetime_fitting_using_the_tcpsc_fitting_
- data_file_import
- o:how_to_measure_the_instrument_response_function_irf|Instrument Response Function (IRF)]] and a decay curve of Coumarin6 dissolved in ethanol. During file ... Original data file content }} Trace #0 shows the IRF, Trace #2 is the decay curve. The other data bloc... any more histograms. It is also possible that an IRF measurement and a decay measurement are stored in
- roi_fitting_using_the_flim_script
- estimation of the instrument response function (=IRF) is shown. The IRF reconstruction is deducted from the rising edge of the images TCSPC histogram. *... } **Response:**\\ * In the TCSPC window, the IRF is displayed in bright red, the data fitting limi... C window. * The new fitting parameters "Shift IRF" and "Bkgr IRF" (=background IRF) appear in the f
- update
- estimation of the instrument response function (=IRF) is shown. The IRF reconstruction is deducted from the rising edge of the [[glossary:TCSPC]] histogram... 00 |}} ** Response:**\\ In the TCSPC window, the IRF is displayed in bright red, the data fitting limi... TCSPC window.\\ The new fitting parameters "Shift IRF" and "Bkgr IRF" (=background IRF) appear in the f
- lifetime-fitting_using_the_rapid_reconvolution_algorithm
- estimation of the instrument response function (=IRF) is shown. The IRF reconstruction is deducted from the rising edge of the [[glossary:TCSPC]] histogram... ill a bit of deviation in the residuals after the IRF in the decay window. This however can be explaine... unchecking them in the fit table, as well as the IRF background and IRF Fit. * One the single pixel
- reconvolution_fit
- an ''.etc'' container that contains at least one IRF histogram and at least one decay curve. Open the ... , we need to select and then assign a curve as an IRF and then change the fitting modality. {{ :howto:e... (selecting) ''crv[0]'', the first histogram, the IRF in this particular case, updates the plot. Now, a... the "Curve type" can be changed from "Decay" to "IRF". {{ :howto:et_fitwindow2.png |}} \\ \\ The p
- flim-fret_calculation_for_single_exponential_donors
- estimation of the instrument response function (=IRF) is shown. The IRF reconstruction is deducted from the rising edge of the TCSPC histogram. On the left... ers τ<sub>D</sub>, Bkgr<sub>Dec</sub>, Shift <sub>IRF</sub> and Bkgr<sub> IRF</sub> constant by removing the mark. Set the background of the decay to 0. *
- visualizing_dynamics_with_the_multi_frame_flim_analysis
- of the instrument response function (= [[glossary:IRF]]) is shown. The [[glossary:IRF]] reconstruction is deducted from the rising edge of the [[glossary:TC
- flim_measurement_using_a_nikon_a1_with_a_flim_and_fcs_upgrade
- :How to measure the Instrument Response function (IRF)]] * [[howto:Recording a Fluorescence Lifetime