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- video_tutorials
- pplications. ==== Fluorescence and Fluorescence Microscopy ==== Fluorescence is the emission of light by a s... p>Fluorescence]]) Fluorescence and Fluorescence Microscopy explained by [[http://www.ibiology.org/ibiosemina... iques/nico-stuurman-part-1.html]] ==== Confocal Microscopy ==== Mark Armitage explains major components of c... njrdLdVa80_N]] \\ Nikons website [[http://www.microscopyu.com/articles/confocal/index.html|MicroscopyU.com
- basics
- {{tag> basics introduction FCS fluorescence microscopy objective resolution NA tutorials}} ====== Basics =====... * [[video_tutorials|Fluorescence and Fluorescence Microscopy by Nico Stuurman]] * [[video_tutorials#Kurt Thorn: Optical Sectioning and Confocal Microscopy|Confocal Microscopy and Optical Sectioning by Kurt Thorn]] * For a complete Video based Microscopy course
- measurement_hardware_instrumentation
- of multiexponetial decays for pure dyes]] ===== Microscopy ===== [[http://www.picoquant.com/images/uploads/... es.pdf|Quick Reference for Confocal Time-resolved Microscopy (FLIM, FRET, FCS)]] - Poster (pdf format) [[video_tutorials|Fluorescence and fluorescence microscopy by Nico Stuurman]] [[video_tutorials#Kurt Thorn: Optical Sectioning and Confocal Microscopy|Confocal microscoy and optical sectioning by Kurt
- community_news @general
- Extremely helpful reference paper about confocal microscopy. [[https://www.nature.com/articles/s41596-020-0313-9|Tutorial: guidance for quantitative confocal microscopy]]. Available as a poster [[https://www.nature.com... 1.20** Exciting work combining FLIM-FRET and STED microscopy in vivo using a newly developed FRET pair! This c... one as revealed by correlative FLIM-FRET and STED microscopy]] \\ \\ * **24.10.19** New publication [[https
- software
- ==== Software ====== ===== Commercial ===== ==== Microscopy ==== [[software:SymPhoTime64]] \\ A complete s... eractive phasor analysis of Fluorescence Lifetime Microscopy (FLIM) data [[https://github.com/LaboratoryOptics... package for quantitative analysis of fluorescence microscopy and spectroscopy data, with a focus on experiment... /github.com/tsbischof/libpicoquant]] * **Open Microscopy's BioFormat** supports PicoQuant bin files ([[glo
- how_to_measure_the_instrument_response_function_irf @howto
- ly measured before or after each measurement. In microscopy-applications, this is usually not practical; and ... recommend to measure the IRF as scatters light in microscopy, due to the color dependence of SPAD detectors, which are generally used in microscopy. Furthermore, even with detectors lacking color e
- start
- scopy]] [[Measurement Hardware / Instrumentation#Microscopy]] ===== Applications ===== /*{{:header-life-scie... }*/ [[Applications#Fluorescence Lifetime Imaging Microscopy (FLIM)]] [[Applications#Foerster Resonance Energ
- applications
- ime Fitting]] ==== Fluorescence Lifetime Imaging Microscopy (FLIM) ==== [[video_tutorials| FLIM by Philippe
- interfacing_time_resolved_spectrometer_fluotime_300_microscope_microtime_100
- {{tag> video FT300 easyTau MT100 FLIM microscopy howto tutorial}} ====== Interfacing a time-resolved spectrom
- lifetime_component_decomposition_phasor_plot_analysis
- **Introduction** Fluorescence lifetime imaging microscopy (FLIM) is a powerful tool for analyzing biologica
- flim @glossary
- M**icroscopy. ([[wp>Fluorescence-lifetime imaging microscopy]]). In FLIM additional image contrast that is ga
- align_beam_backreflection @howto
- {{tag>howto MicroTime200 microscopy backreflection alignment }} ====== Image on the beam diagnostics camera du
- exchange_dichroic_mt200 @howto
- {{tag>howto MicroTime200 dichroic microscopy}} ====== How to exchange the main dichroic of the MicroTime 200
- flim-fret_calculation_for_single_exponential_donors @howto
- |Fluorescence Lifetime Imaging (FLIM) in Confocal Microscopy Applications: An Overview, available from PicoQua