Search
You can find the results of your search below.
Matching pagenames:
Fulltext results:
- software
- nfiguration with PicoQuant Time Correlated Single Photon Counting Instruments (TCSPC devices). It harnesse... sampled” signals or on “point-processes”, such as photon timestamps. Pycorrelate allows computing cross-co... on to support the use of a Time-Correlated Single Photon Counting system for application to Positron Annih... Python package to analyze time-correlated single-photon counting (TCSPC) data sets, namely fluorescence l
- pile-up_effect @glossary
- ect describes the effects of photons lost at high photon count rates due to the dead time of the TCSPC devices. Most single photon counting detectors and [[TCSPC]] electronics have a [[dead time]]. After registering one photon the device needs some time to be ready for the next photon to detect. The dead time for TCSPC devices used f
- lifetime-fitting_using_the_rapid_reconvolution_algorithm @howto
- s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... ence Lifetime Histogram**\\ Here the frequency of photon counts corresponding to the individual mean lifet... ntrols on the right of the plot. The histogram is photon weighted.\\ **4: Decay Fitting Parameters**\\ Her... decay due to limited pulse pair resolution of the photon detector; Even the best detector cannot deliver i
- differential_count_rate @glossary
- /Differential count rate// is the real, momentary photon density (probability of encountering a photon) during a time interval when a detected signal is really pr... rential count rate if we are detecting rarely one photon (count) per excitation cycle, anyway? Detection p... flashes. These are short time intervals with huge photon density (rate), separated by long "dark", quiet p
- t3-mode @glossary
- cord them only if they arrive in the context of a photon event on channel 1. The event record is then com... - The start-stop timing difference between the photon event and the last sync event, and - the arriva... ssible to precisely determine which sync period a photon event belongs to. Since the sync period is also k... ore allows to reconstruct the arrival time of the photon with respect to the overall experiment time. T3
- antibunching_measurements @howto
- photons emitted (after the detection of a genuine photon) by one of the SPADs, detected by the second SPAD... o H. C., Huang J., Tang J.: //Antibunching single-photon emission and blinking suppression of CdSe/ZnS qua... ting M., Wahl M., Erdmann R: //Recent advances in photon coincidence measurements for photon antibunching and full correlation analysis//. In: //Proceedings of SP
- video_tutorials
- es/kurt-thorn-part-1.html]] ==== Kurt Thorn: Two Photon Microscopy ==== Kurt Thorn of UCSF talks about two-photon microscopy which uses intense pulsed lasers to im... www.ibiology.org/ibioeducation/taking-courses/two-photon-microscopy.html]] ==== Philippe Bastiaens: Fluo... an imaging technique in confocal microscopy, two-photon excitation microscopy, and multiphoton tomography
- flim_fret_calculation_for_multi_exponential_donors @howto
- s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... escence Lifetime Histogram. Here the frequency of photon counts corresponding to the individual mean lifet... electronics (e.g. hybrid PMA-detector + MultiHarp photon counting board), where the peak count rates have ... s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve
- measurement_hardware_instrumentation
- dth analysis]] [[video_tutorials#Kurt Thorn: Two Photon Microscopy| Two Photon Microscopy (TPM, TPE) by Kurt Thorn]] [[general:advantages_and_disadvantages_of_two_photon_excitation_tpe|Advantages and disadvantages of Two Photon Excitation (TPE)]] [[howto:how_to_measure_the_in
- lifetime-fitting_using_the_flim_script @howto
- s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... ence Lifetime Histogram**\\ Here the frequency of photon counts corresponding to the individual mean lifet... ntrols on the right of the plot. The histogram is photon weighted.\\ **4: Decay Fitting Parameters**\\ Her... f the decay window (often, this is the case for 2-Photon-Excitation data acquired with a TiSa-laser with 8
- using_the_antibunching_script @howto
- easurements of NV centers which are stable single photon sources excitable at 530 nm. The script is used t... of the complete trace above marked in green. The photon counting histogram on the right displays the freq... his corresponds to the fact that if just a single photon is emitted, it cannot be registered by both detec... ves are calculated, as for the calculation of the photon pairs, detector 1 or 2 can be used as reference.
- visualizing_dynamics_using_the_multiframe-flim_script @howto
- s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... ence Lifetime Histogram**\\ Here the frequency of photon counts corresponding to the individual mean lifet... ntrols on the right of the plot. The histogram is photon weighted.\\ **4: Decay Fitting Parameters**\\ Her... in order to decrease processing time and increase photon numbers per pixel, while still visualizing the te
- advantages_and_disadvantages_of_two_photon_excitation_tpe @general
- ====== Advantages and disadvantages of two photon excitation (TPE) ====== *The main advantage of two photon excitation is a deeper penetration and less photo... *The absorption is much less compared to one photon excitation. This leads very often to high intensi... al. ===== Laser Safety ===== Lasers used for 2 Photon Excitation are usually laser class IV. Laser Clas
- fluorescence_correlation_spectroscopy-_a_short_introduction @general
- S0-S1 excitation) and cannot emit a fluorescence photon. {{:general:singlet-triplet-fluctuations.png|}} ... tion spectroscopy by using time-correlated single photon counting. \\ Triffo et al, 2012, "Monitoring Lipi... s of proteome dynamics in living cells" * **two-photon FCS** \\ Schwille and Heinze, 2001, "Two-Photon cross-correlation spectroscopy" * **scanning FCS** \\ R
- lifetime_fitting_using_the_flim_analysis @howto
- s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... escence Lifetime Histogram. Here the frequency of photon counts corresponding to the individual mean lifet... exity of the model. In order to check whether the photon number is sufficient for the model suggested, cli... * Move the mouse around the image and read the photon numbers. Inside the pollen, there are about 5000