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- flim_fret_calculation_for_multi_exponential_donors @howto
- LIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwis... ximum. As the mean arrival time of the background photons in the areas where no fluorescence is detected, ... s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... escence Lifetime Histogram. Here the frequency of photon counts corresponding to the individual mean lifet
- roi_fitting_using_the_flim_script @howto
- LIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwis... ximum. As the mean arrival time of the background photons in the areas where no fluorescence is detected, ... s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... escence Lifetime Histogram. Here the frequency of photon counts corresponding to the individual mean lifet
- software
- nfiguration with PicoQuant Time Correlated Single Photon Counting Instruments (TCSPC devices). It harnesse... sampled” signals or on “point-processes”, such as photon timestamps. Pycorrelate allows computing cross-co... on to support the use of a Time-Correlated Single Photon Counting system for application to Positron Annih... Python package to analyze time-correlated single-photon counting (TCSPC) data sets, namely fluorescence l
- lifetime_fitting_using_the_flim_analysis @howto
- LIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwis... ximum. As the mean arrival time of the background photons in the areas where no fluorescence is detected, ... s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... escence Lifetime Histogram. Here the frequency of photon counts corresponding to the individual mean lifet
- pile-up_effect @glossary
- ===== The Pile-Up effect describes the effects of photons lost at high photon count rates due to the dead time of the TCSPC devices. Most single photon counting detectors and [[TCSPC]] electronics have a [[dead time]]. After registering one photon the device needs some time to be ready for the ne
- lifetime-fitting_using_the_rapid_reconvolution_algorithm @howto
- LIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwis... ximum. As the mean arrival time of the background photons in the areas where no fluorescence is detected, ... s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... ence Lifetime Histogram**\\ Here the frequency of photon counts corresponding to the individual mean lifet
- differential_count_rate @glossary
- /Differential count rate// is the real, momentary photon density (probability of encountering a photon) during a time interval when a detected signal is really pr... rential count rate if we are detecting rarely one photon (count) per excitation cycle, anyway? Detection probability of two or more photons in one cycle is completely negligible!//" Not r
- lifetime-fitting_using_the_flim_script @howto
- LIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwis... ximum. As the mean arrival time of the background photons in the areas where no fluorescence is detected, ... s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... ence Lifetime Histogram**\\ Here the frequency of photon counts corresponding to the individual mean lifet
- antibunching_measurements @howto
- of the dip, they are however artifacts caused by photons emitted (after the detection of a genuine photon) by one of the SPADs, detected by the second SPAD. The ... o H. C., Huang J., Tang J.: //Antibunching single-photon emission and blinking suppression of CdSe/ZnS qua... ting M., Wahl M., Erdmann R: //Recent advances in photon coincidence measurements for photon antibunching
- visualizing_dynamics_using_the_multiframe-flim_script @howto
- LIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwis... ximum. As the mean arrival time of the background photons in the areas where no fluorescence is detected, ... s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... ence Lifetime Histogram**\\ Here the frequency of photon counts corresponding to the individual mean lifet
- t3-mode @glossary
- cord them only if they arrive in the context of a photon event on channel 1. The event record is then com... - The start-stop timing difference between the photon event and the last sync event, and - the arriva... ssible to precisely determine which sync period a photon event belongs to. Since the sync period is also k... ore allows to reconstruct the arrival time of the photon with respect to the overall experiment time. T3
- symphotime_tips_and_tricks @howto
- eflim-1.png?300|}}</imgcaption> </WRAP> * Max. Photons (in the brightest pixel for the selected data ch... into account the pixel recording times) * Avg. Photons (in a pixel) * Avg. Countrate (calculated taki... . Counts === TCSPC === * Max. Counts (number of photons in the peak TCSPC-channel) You can also add add... unbinned image, which calculates the mean of the photon arrival times, you will notice that the lifetime
- visualizing_dynamics_with_the_multi_frame_flim_analysis @howto
- LIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwis... ximum. As the mean arrival time of the background photons in the areas where no fluorescence is detected, ... read over the [[glossary:TCSPC]] window, the mean photon arrival time of the dark background is usually ve... escence Lifetime Histogram. Here the frequency of photon counts corresponding to the individual mean lifet
- advantages_and_disadvantages_of_two_photon_excitation_tpe @general
- ====== Advantages and disadvantages of two photon excitation (TPE) ====== *The main advantage of two photon excitation is a deeper penetration and less photo... *The absorption is much less compared to one photon excitation. This leads very often to high intensi... er: You will get just ten times less fluorescence photons. This leads to limitations for FCS and other sin
- update @howto
- LIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwis... ximum. As the mean arrival time of the background photons in the areas where no fluorescence is detected, ... s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... ence Lifetime Histogram**\\ Here the frequency of photon counts corresponding to the individual mean lifet