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- flim_fret_calculation_for_multi_exponential_donors
- LIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwis... ximum. As the mean arrival time of the background photons in the areas where no fluorescence is detected, ... s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... escence Lifetime Histogram. Here the frequency of photon counts corresponding to the individual mean lifet
- roi_fitting_using_the_flim_script
- LIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwis... ximum. As the mean arrival time of the background photons in the areas where no fluorescence is detected, ... s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... escence Lifetime Histogram. Here the frequency of photon counts corresponding to the individual mean lifet
- lifetime_fitting_using_the_flim_analysis
- LIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwis... ximum. As the mean arrival time of the background photons in the areas where no fluorescence is detected, ... s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... escence Lifetime Histogram. Here the frequency of photon counts corresponding to the individual mean lifet
- lifetime-fitting_using_the_rapid_reconvolution_algorithm
- LIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwis... ximum. As the mean arrival time of the background photons in the areas where no fluorescence is detected, ... s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... ence Lifetime Histogram**\\ Here the frequency of photon counts corresponding to the individual mean lifet
- lifetime-fitting_using_the_flim_script
- LIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwis... ximum. As the mean arrival time of the background photons in the areas where no fluorescence is detected, ... s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... ence Lifetime Histogram**\\ Here the frequency of photon counts corresponding to the individual mean lifet
- antibunching_measurements
- of the dip, they are however artifacts caused by photons emitted (after the detection of a genuine photon) by one of the SPADs, detected by the second SPAD. The ... o H. C., Huang J., Tang J.: //Antibunching single-photon emission and blinking suppression of CdSe/ZnS qua... ting M., Wahl M., Erdmann R: //Recent advances in photon coincidence measurements for photon antibunching
- visualizing_dynamics_using_the_multiframe-flim_script
- LIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwis... ximum. As the mean arrival time of the background photons in the areas where no fluorescence is detected, ... s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... ence Lifetime Histogram**\\ Here the frequency of photon counts corresponding to the individual mean lifet
- symphotime_tips_and_tricks
- eflim-1.png?300|}}</imgcaption> </WRAP> * Max. Photons (in the brightest pixel for the selected data ch... into account the pixel recording times) * Avg. Photons (in a pixel) * Avg. Countrate (calculated taki... . Counts === TCSPC === * Max. Counts (number of photons in the peak TCSPC-channel) You can also add add... unbinned image, which calculates the mean of the photon arrival times, you will notice that the lifetime
- visualizing_dynamics_with_the_multi_frame_flim_analysis
- LIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwis... ximum. As the mean arrival time of the background photons in the areas where no fluorescence is detected, ... read over the [[glossary:TCSPC]] window, the mean photon arrival time of the dark background is usually ve... escence Lifetime Histogram. Here the frequency of photon counts corresponding to the individual mean lifet
- update
- LIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwis... ximum. As the mean arrival time of the background photons in the areas where no fluorescence is detected, ... s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... ence Lifetime Histogram**\\ Here the frequency of photon counts corresponding to the individual mean lifet
- using_the_antibunching_script
- easurements of NV centers which are stable single photon sources excitable at 530 nm. The script is used t... of the complete trace above marked in green. The photon counting histogram on the right displays the freq... his corresponds to the fact that if just a single photon is emitted, it cannot be registered by both detec... ves are calculated, as for the calculation of the photon pairs, detector 1 or 2 can be used as reference.
- flim-fret_calculation_for_single_exponential_donors
- FLIM" lifetime, i.e. the mean arrival time of the photons after the laser pulse. When not defined otherwis... ximum. As the mean arrival time of the background photons in the areas where no fluorescence is detected, ... s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... . - **Lower center/left: TCSPC histogram of all photons in the image.** In green (= dataset 0) and the T
- t3r_antibunching_-_slow_decay
- happens because you correlate the first arriving photon against a photon at the longer time, which will be an increasingly unlikely event because the earliest stop photons always win and the experiment restarts. A simul... art-stop correlation but the correlation of every photon against every photon. It can be done using the S
- calculate_and_fit_fcs_traces_with_the_fcs_script
- of the complete trace above marked in green. The photon counting histogram on the right displays the freq... the laser pulse on the time - range of interest, photons caused by detector afterpulsing behave similar as normal background photons. **Note:** If you want to check what is calcula... essary to calculate first a FCS curve without the photon filter. You can also directly activate the FLCS b
- calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script
- pt_Image_10.png }} **Note:** The parameter "Min. Photons per FRET Event" is by default set to 25. In orde... ciencies are otherwise too much determined by the photon numbers of the event. For example, in a burst with just 10 photons, only 11 FRET values are numerically possible, w... nts without ("Classical FRET") and with PIE based photon filtering ("PIE-FRET") are plotted. * Below t