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- lifetime-fitting_using_the_rapid_reconvolution_algorithm
- s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... ence Lifetime Histogram**\\ Here the frequency of photon counts corresponding to the individual mean lifet... ntrols on the right of the plot. The histogram is photon weighted.\\ **4: Decay Fitting Parameters**\\ Her... decay due to limited pulse pair resolution of the photon detector; Even the best detector cannot deliver i
- antibunching_measurements
- photons emitted (after the detection of a genuine photon) by one of the SPADs, detected by the second SPAD... o H. C., Huang J., Tang J.: //Antibunching single-photon emission and blinking suppression of CdSe/ZnS qua... ting M., Wahl M., Erdmann R: //Recent advances in photon coincidence measurements for photon antibunching and full correlation analysis//. In: //Proceedings of SP
- flim_fret_calculation_for_multi_exponential_donors
- s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... escence Lifetime Histogram. Here the frequency of photon counts corresponding to the individual mean lifet... electronics (e.g. hybrid PMA-detector + MultiHarp photon counting board), where the peak count rates have ... s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve
- lifetime-fitting_using_the_flim_script
- s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... ence Lifetime Histogram**\\ Here the frequency of photon counts corresponding to the individual mean lifet... ntrols on the right of the plot. The histogram is photon weighted.\\ **4: Decay Fitting Parameters**\\ Her... f the decay window (often, this is the case for 2-Photon-Excitation data acquired with a TiSa-laser with 8
- using_the_antibunching_script
- easurements of NV centers which are stable single photon sources excitable at 530 nm. The script is used t... of the complete trace above marked in green. The photon counting histogram on the right displays the freq... his corresponds to the fact that if just a single photon is emitted, it cannot be registered by both detec... ves are calculated, as for the calculation of the photon pairs, detector 1 or 2 can be used as reference.
- visualizing_dynamics_using_the_multiframe-flim_script
- s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... ence Lifetime Histogram**\\ Here the frequency of photon counts corresponding to the individual mean lifet... ntrols on the right of the plot. The histogram is photon weighted.\\ **4: Decay Fitting Parameters**\\ Her... in order to decrease processing time and increase photon numbers per pixel, while still visualizing the te
- lifetime_fitting_using_the_flim_analysis
- s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... escence Lifetime Histogram. Here the frequency of photon counts corresponding to the individual mean lifet... exity of the model. In order to check whether the photon number is sufficient for the model suggested, cli... * Move the mouse around the image and read the photon numbers. Inside the pollen, there are about 5000
- t3r_antibunching_-_slow_decay
- happens because you correlate the first arriving photon against a photon at the longer time, which will be an increasingly unlikely event because the earliest st... art-stop correlation but the correlation of every photon against every photon. It can be done using the SymPhoTime Software (http://www.picoquant.com/products/sw
- update
- s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... ence Lifetime Histogram**\\ Here the frequency of photon counts corresponding to the individual mean lifet... ntrols on the right of the plot. The histogram is photon weighted.\\ **4: Decay Fitting Parameters**\\ Her
- visualizing_dynamics_with_the_multi_frame_flim_analysis
- read over the [[glossary:TCSPC]] window, the mean photon arrival time of the dark background is usually ve... escence Lifetime Histogram. Here the frequency of photon counts corresponding to the individual mean lifet... ing of 3 to decrease processing time and increase photon numbers per pixel. {{ visualizing_dynamics_Image_
- calculate_and_fit_fcs_traces_with_the_fcs_script
- of the complete trace above marked in green. The photon counting histogram on the right displays the freq... essary to calculate first a FCS curve without the photon filter. You can also directly activate the FLCS b
- calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script
- ciencies are otherwise too much determined by the photon numbers of the event. For example, in a burst wit... nts without ("Classical FRET") and with PIE based photon filtering ("PIE-FRET") are plotted. * Below t
- flim-fret_calculation_for_single_exponential_donors
- s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... Response:** In the image, only pixels with higher photon counts are highlighted. Adapt the intensity maxim
- how_to_measure_the_instrument_response_function_irf
- for fluorescence measurement </code> ==== Two photon excitation (TPE) ==== Do not attempt to record a... an be adapted. A special case are systems with 2-Photon-Excitation (2PE). Here, usually TiSa-lasers are u
- intensity_time_trace_analysis
- r trace for the two channels.\\ **On the right**, photon counting histograms (PCH) are plotted, indicating... arker Signals in Images ==== **Note:** PicoQuant photon counting boards can register up to four external