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avoid_pile_up_effect_in_flim_measurements @howto
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{{tag> howto FLIM acquisition pile-up TCSPC}} ~~TOC~~ ====== How to Avoid the Pile-up Effect in FLIM Measurements ====== For a definition of the pile-up effect see here: [[glossary:pile-up_effect|Pile-Up Effect]] To avoid [[glossary:pile-up_effect|pile
pile-up_effect @glossary
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{{tag> TCSPC pile-up dead_time}} ~~TOC~~ ====== Pile-Up Effect ====== The Pile-Up effect describes the effects of photons lost at high photon count rates du... can detect only one photon after one laser shot. "Pile up" describes the effects of photons lost at high
differential_count_rate @glossary
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{{tag> pile-up dead_time IRF differential_countrate}} ====== Differential Count Rate ====== Ideally, a... void various counting losses.(E.g. to avoid the [[pile-up effect]]). However, for an IRF measurement the
dead_time @glossary
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l be lost. This leads to the so called [[glossary:Pile-up Effect]]. Note that not only TCSPC devices bu
flim_fret_calculation_for_multi_exponential_donors @howto
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unt rates have been significantly higher than the pile up limit, or in cases, where the repetition rate
lifetime-fitting_using_the_flim_script @howto
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he image was acquired are significantly above the Pile Up limit. It is also needed, if the pulse frequen
lifetime-fitting_using_the_rapid_reconvolution_algorithm @howto
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le hardware with count-rates way beyond classical pile up limit. For the principle of rapidFLIM - reco
update @howto
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a system with a dead time significantly above the pile-up-limit or when the image has been acquired with