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- symphotime_tips_and_tricks
- ile|<WRAP> - Generated from the online analysis selected during the measurement (the Countrate-Display i... the values calculated during an online analysis, select the "**Comment**"-tab. The kind and amount of t... * Max. Photons (in the brightest pixel for the selected data channel) * Max. Countrate (calculated ta... ween the different display options can be done by selecting the desired display option above the scale. ^
- update
- rmed.\\ ===== Step-by-step Tutorial ===== ==== Select a file and start the analysis ==== * Start Sym... ey_Cells_FLIM.ptu'' by a single mouse click. * Select the **"Analysis"** tab and in there, open the *... meters**\\ Here the lifetime fitting model can be selected. Fitting is then applied to the lifetime graph(... st by adapting "Min" and "Max" values. ==== Select a file and start the analysis ==== * In the FL
- roi_fitting_using_the_flim_script
- vLQW4nY?large }} ===== Step-by-Step ===== ==== Select a file and start the script ==== * Start [[pro... fitting_using_the_flim_script_Image_2.png }} * Select the "Analysis"-tab and in there, open the drop do... ime contrasts in the pollen become visible. ==== Select 3 Regions of Interest (ROI) ==== * Keep the m... ing_using_the_flim_script_Image_7.png?349 }} * Select a ROI tool in the context menu, e.g. the "FreeROI
- lifetime-fitting_using_the_rapid_reconvolution_algorithm
- 6c23]]. ===== Step-by-Step Tutorial ===== ==== Select a file and start the analysis ==== * Start Sym... 'MicroBeads.ptu'' by a single mouse click. * Select the **"Analysis"** tab and in there, open the **"... meters**\\ Here the lifetime fitting model can be selected. Fitting is then applied to the lifetime graph(... t by adapting "Min" and "Max" values. ==== Select ROIs ==== * On top left, under Region of Int
- pattern_matching
- nd an overall decay from the raw data file. ==== Select Patterns ==== **Note:** Once an image is generated, the next step is to select the patterns which are to be used for decomposing... g_Image_7.png?600 }} * In the right-click menu select the magic wand ROI (region-of-interest). {{ pattern_matching_Image_8.png?600 }} * Select the innermost contour line by clicking on it. {{
- flim_fret_calculation_for_multi_exponential_donors
- for_multi-exponential_donors_Image_2.png? }} * Select the "Analysis" tab and in there, open the drop do... and open the context menu by a right mouse click. Select the "Free ROI" tool. {{ flim-fret-calculation_for... **\\ For more information about the different ROI selection tools, check the tutorial [[howto:roi_fitting_... he left mouse button activated while circling the selected part of the image. Then release the left mouse
- visualizing_dynamics_using_the_multiframe-flim_script
- f86c23]]. ===== Step-by-Step Tutorial ===== ==== Select a file and start the analysis ==== * Start Sym... ck_MDCK_Cells.ptu'' by a single mouse click. * Select the **"Analysis"** tab and in there, open the *... meters**\\ Here the lifetime fitting model can be selected. Fitting is then applied to the lifetime graph(... ram.\\ ==== Define image stack options and select ROI ==== * In parameter panel, enter a frame
- lifetime-fitting_using_the_flim_script
- formed. ===== Step-by-step Tutorial ===== ==== Select a file and start the analysis ==== * Start SymP... ey_Cells_FLIM.ptu'' by a single mouse click. * Select the **"Analysis"** tab and in there, open the *... meters**\\ Here the lifetime fitting model can be selected. Fitting is then applied to the lifetime graph(... st by adapting "Min" and "Max" values. ==== Select ROIs ==== * Open the "Region of Interest" drop
- antibunching_measurements
- rep. rate and figure 2 (40 MHz) rep rate). *Select [[glossary:T2-mode]] *Start Point Measureme... kcps. ==== Analysis in SymPhoTime ==== *Select FCS Analysis * Select //CrossCorrelation// and //Antibunching// *//Left Correlation Channel:// *[[Products:PicoHarp 300]]: Select //Ch0// (this is now SPAD 1) *[[Products:
- lifetime_fitting_using_the_tcpsc_fitting_script
- This tutorial shows step-by-step, how to fit a selected model to a decay curve in order to get the life... VV8?large }} ===== Step-by-Step Tutorial ===== Select a file and start the script * Start [[products... sing_the_tcpsc_fitting_script_Image_2.png }} * Select the "Analysis" tab and in there, open the drop do... ured sample. * In the decay form on the left, select n-exponential reconvolution as fitting model. **R
- separation_of_2_species_with
- d laser. ===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[pro... separation_of_2_species_with_Image_2.png }} * Select the "Analysis" tab and in there, open the drop do... as evenly splitted onto two detectors. Therefore, select both detectors in the definition of "Channel A": ... ration_of_2_species_with_Image_10.png?383 }} * Select the file ''Cy5_diff_IRF+FLCS-pattern.ptu'' and cl
- using_the_anisotropy_image_script
- on. \\ ===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[pro... g_the_anisotropy_image_script_Image_2.png }} * Select the "Analysis" tab and in there, open the drop do... measuring a randomly aligned ensemble due to photoselection the anisotropy varies with in the limits of -... ivate the context menu by a right mouse click and select "Show data reader". The intensity of the pixel wh
- intensity_time_trace_analysis
- }} \\ ===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[pro... e_intensity_time_trace_script_Image_2.png }} * Select the "Analysis" tab and in there, open the drop do... en calculated yet, this area is still empty. * Select a "Binning" of 10 ms. * Click "Calculate". {{ ... Image_6.png }} **Response:** As activated under "Select data channels", two intensity plots are calculate
- visualizing_dynamics_with_the_multi_frame_flim_analysis
- large }} ===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[pro... lick. {{ visualizing_dynamics_Image_2.png }} * Select the "Analysis" tab and in there, open the drop do... cess indicated by blurring of certain GUVs. ==== Select the Processing Options and create and export an i... ual frames. By default, a frame binning of 102 is selected. Thus, the measurement is plotted as one image
- calculate_ratiometric_single_pair_fret_distributions
- \\ \\ ===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[pro... ingle_pair_fret_distributions_Image_2.png }} * Select the "Analysis" tab and in there, open the drop do... ms. **Note:** If you are unsure which binning to select, calculate the FCS curve and check the diffusion ... ivate the context menu by a right mouse click and select "Show Data Reader/Select Curve". {{ calculate_ra