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- roi_fitting_using_the_flim_script @howto
- vLQW4nY?large }} ===== Step-by-Step ===== ==== Select a file and start the script ==== * Start [[pro... fitting_using_the_flim_script_Image_2.png }} * Select the "Analysis"-tab and in there, open the drop do... ime contrasts in the pollen become visible. ==== Select 3 Regions of Interest (ROI) ==== * Keep the m... ing_using_the_flim_script_Image_7.png?349 }} * Select a ROI tool in the context menu, e.g. the "FreeROI
- lifetime-fitting_using_the_rapid_reconvolution_algorithm @howto
- 6c23]]. ===== Step-by-Step Tutorial ===== ==== Select a file and start the analysis ==== * Start Sym... 'MicroBeads.ptu'' by a single mouse click. * Select the **"Analysis"** tab and in there, open the **"... t by adapting "Min" and "Max" values. ==== Select ROIs ==== * On top left, under Region of Int... d Fast Lifetime color code. This will help you to select your ROI or threshold easier. {{ howto:adaptcont
- visualizing_dynamics_using_the_multiframe-flim_script @howto
- wto:roi0_10framebinning_2.png |}} * Tick the "Select in all Frames" checkbox. Click "ROI per Frame" to select an ROI with the same marked pixels in each frame. \\ {{ howto:select_in_all_frames.png?300 |}} **Response:**\\ When ... ing" panel, from the Fitting Model drop-down list select "n-Exponential Reconvolution Fit".\\ {{ howto:ne... t parameters.\\ * From "Decay" drop-down list select ''ROI 0'' and click "Initial Fit" (marked in oran
- flim_fret_calculation_for_multi_exponential_donors @howto
- for_multi-exponential_donors_Image_2.png? }} * Select the "Analysis" tab and in there, open the drop do... and open the context menu by a right mouse click. Select the "Free ROI" tool. {{ flim-fret-calculation_for... 0 }} * In the decay form on the lower left, select n-exponential reconvolution as fitting model. {{ ... of the different parameters. * Under "Decay" select "ROI 0". **Response:**\\ The TCSPC curve of ROI 0
- update @howto
- nu.\\ - If you want to start again from scratch select "Select all as ROI".\\ * In this step, as an example, ... pixels with similar fast lifetime. Ideally try to select an ROI with 10000 to 100000 counts in peak for ge... nents might not be resolved.\\ * In order to select an additional ROI, click "New" ROI.\\ {{ howto:a... g |}} * Repeat the last two steps in order to select the cell nuclei as the last ROI.\\ {{ howto:nucl
- symphotime_tips_and_tricks @howto
- ion. * If you want to start from scretch again, select **“Select all as ROI”** * you can also invert the select... _gray.png?300|}} | Now use the drop-down list to select the parameter used for the "gray-scale" and select "FastLT" (or any other parameter of your choice)... cay and the residuals as shown, it is possible to select, which cells should be exported. {{graph_exporto
- antibunching_measurements @howto
- 400 select Ch2 (SPAD 2) *//LagTime To:// select few multiples of the Laser Repetition Rate (e.g. for 10 MHz select 700 ns – so you will see 7 peaks) *//Delt
- lifetime-fitting_using_the_flim_script @howto
- the fourth box, "Decay Fitting", below the image, select the Fitting Model drop-down list select "n-Exponential Reconvolution Fit".\\ {{ howto:ne... a measured IRF, press on "Import" IRF button and select the corresponding file from the file list (make s... measured IRF.\\ * From "Decay" drop-down list select ''ROI 0'' and click "Initial Fit" (marked in oran... ed false color scale.\\ * In RGB mode you can select suited color to your favorite parameter. This hel
- pattern_matching @howto
- tching_Image_23.png }} * In the pop-up window, select the reference-measurement (in the example select //''non-FRET sample.ptu''//) and confirm by click... ribed above, but instead of "non-FRET sample.ptu" select the created pattern (''Pat_non-FRET sample.pres''... tterns. {{ pattern_matching_Image_27.png }} * Select the //Free ROI// tool in the right-click menu in
- separation_of_2_species_with @howto
- d laser. ===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[pro... separation_of_2_species_with_Image_2.png }} * Select the "Analysis" tab and in there, open the drop do... as evenly splitted onto two detectors. Therefore, select both detectors in the definition of "Channel A": ... ration_of_2_species_with_Image_10.png?383 }} * Select the file ''Cy5_diff_IRF+FLCS-pattern.ptu'' and cl
- calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script @howto
- ivate the context menu by a right mouse click and select "Show Data Reader/Select Curve". {{ calculate_ratiometric_single_pair_fre... _Image_20.png }} **Response:** A window opens to select the user settings file. Select the recently stored file. {{ calculate_ratiometri
- intensity_time_trace_analysis @howto
- }} \\ ===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[pro... e_intensity_time_trace_script_Image_2.png }} * Select the "Analysis" tab and in there, open the drop do... en calculated yet, this area is still empty. * Select a "Binning" of 10 ms. * Click "Calculate". {{ ... Image_6.png }} **Response:** As activated under "Select data channels", two intensity plots are calculate
- calculate_ratiometric_single_pair_fret_distributions @howto
- ivate the context menu by a right mouse click and select "Show Data Reader/Select Curve". {{ calculate_ratiometric_single_pair_fre... _Image_18.png }} **Response:** A window opens to select the user settings file. Select the recently stored file. {{ calculate_ratiometri
- using_the_anisotropy_image_script @howto
- _Image_22.png }} **Response:** A window opens to select the user settings file. Select the recently generated file. {{ using_the_anisotr
- lifetime_fitting_using_the_tcpsc_fitting_script @howto
- VV8?large }} ===== Step-by-Step Tutorial ===== Select a file and start the script * Start [[products... sing_the_tcpsc_fitting_script_Image_2.png }} * Select the "Analysis" tab and in there, open the drop do... ured sample. * In the decay form on the left, select n-exponential reconvolution as fitting model. **R... a right mouse click to open the context menu and select "Copy" or simple use "<CRTL>" + "C" to copy the f