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- symphotime_tips_and_tricks @howto
- ou want to remove some of the pixels from the ROI selection, just keep the ''<CTRL>'' - key pressed when using a ROI selection tool to unselect unneeded pixels from the sele... ion. * If you want to start from scretch again, select **“Select all as ROI”** * you can also invert the select... _gray.png?300|}} | Now use the drop-down list to select the parameter used for the "gray-scale" and select "FastLT" (or any other parameter of your choice)... mage according to "FastLT" and by clicking on the selected line the graph in the lower panel is updated an
- update @howto
- If you want to remove some of the pixels from the selected ROI, just keep the ''<CTRL>'' key pressed when using a ROI selection tool to exclude the unwanted parts.\\ - Addi... nu.\\ - If you want to start again from scratch select "Select all as ROI".\\ * In this step, as an example, ... image with a similar fast lifetime range will be selected and the lifetime histogram will get updated acc... as it's done here, you have to press "Shift" when selecting the spatial separated areas with the Magic wan
- roi_fitting_using_the_flim_script @howto
- rked ROI, keep the cursor over the image with the selected ROI, open the context menu with a right mouse click and select one of the export options:\\ **Bitmap** exports t... e raw image. ==== Perform a Lifetime Fit on the Selected Regions (ROIs) ==== * In the decay form on the lower left, select n-exponential reconvolution as fitting model. {{ ... equations, click on the "Help" button next to the selected model. This opens a help window containing the
- lifetime-fitting_using_the_rapid_reconvolution_algorithm @howto
- 6c23]]. ===== Step-by-Step Tutorial ===== ==== Select a file and start the analysis ==== * Start Sym... 'MicroBeads.ptu'' by a single mouse click. * Select the **"Analysis"** tab and in there, open the **"... meters**\\ Here the lifetime fitting model can be selected. Fitting is then applied to the lifetime graph(... t by adapting "Min" and "Max" values. ==== Select ROIs ==== * On top left, under Region of Int
- pattern_matching @howto
- tching_Image_23.png }} * In the pop-up window, select the reference-measurement (in the example select //''non-FRET sample.ptu''//) and confirm by click... ains only the desired component. To make a better selection of the pattern to be imported, open the extern... extract the desired non-FRET pattern. * After selecting this pattern, click Save in the Patterns box. ... ribed above, but instead of "non-FRET sample.ptu" select the created pattern (''Pat_non-FRET sample.pres''
- flim_fret_calculation_for_multi_exponential_donors @howto
- for_multi-exponential_donors_Image_2.png? }} * Select the "Analysis" tab and in there, open the drop do... and open the context menu by a right mouse click. Select the "Free ROI" tool. {{ flim-fret-calculation_for... **\\ For more information about the different ROI selection tools, check the tutorial [[howto:roi_fitting_... he left mouse button activated while circling the selected part of the image. Then release the left mouse
- burst_duration_and_fcs_diffusion_time @howto:burst_duration_and_fcs_diffusion_time
- CS diffusion time ====== **Diffusion, flow, burst selection, and photobleaching in confocal single-molecul... ntly to transport mechanisms, detection geometry, selection criteria, and photophysical processes. As a co... ion conditions. Finally, we investigate how burst-selection procedures, directed flow, and photobleaching ... ear spatial weighting, modified by explicit event selection. Short, peripheral trajectories often do not e
- create_time_gated_image
- in the main control window (in fact, you can also select the time gate there, but as the window is smaller, it's the selection is not so precise. {{:flim-image_after_gate_s... png|}} * If a second time-gated image should be selected, either just select a new time gate as described above and repeat the... nd time-gated image with the complementary photon selection, just click on the white area in the gate-selection graph and click "fast-FLIM" again. Then you can select the grey-scale instead of the rainbow scale to di
- visualizing_dynamics_using_the_multiframe-flim_script @howto
- wto:roi0_10framebinning_2.png |}} * Tick the "Select in all Frames" checkbox. Click "ROI per Frame" to select an ROI with the same marked pixels in each frame. \\ {{ howto:select_in_all_frames.png?300 |}} **Response:**\\ When ... |}} ==== Perform an Initial Lifetime Fit on the Selected Regions (ROIs) ==== * In the "Decay Fitting" panel, from the Fitting Model drop-down list select "n-Exponential Reconvolution Fit".\\ {{ howto:ne
- lifetime-fitting_using_the_flim_script @howto
- of the image.\\ * When you are content with the selection, press OK to select these pixels of the image as Region of Interest (... the fourth box, "Decay Fitting", below the image, select the Fitting Model drop-down list select "n-Exponential Reconvolution Fit".\\ {{ howto:ne... equations, click on the "Help" button next to the selected model. This opens a help window containing the ... a measured IRF, press on "Import" IRF button and select the corresponding file from the file list (make s
- antibunching_measurements @howto
- 400 select Ch2 (SPAD 2) *//LagTime To:// select few multiples of the Laser Repetition Rate (e.g. for 10 MHz select 700 ns – so you will see 7 peaks) *//Delt... tion. *e.g. for 32 ps Resolution Setting, selecting a Delta = 128 will result in approx. 4 ns step... fluorescence photon antibunching IEEE Journal of Selected Topics//. In: //Quantum Electronics//. Vol.13,
- lifetime_fitting_using_the_tcpsc_fitting_script @howto
- he right mouse click to open the context menu and select one of the ASCII export options, in this case "selected cell". {{ lifetime_fitting_using_the_tcpsc_fitt... or a file name to store the exported result file. Select e.g. the name ''decay.dat''. {{ lifetime_fitting_... ate the context menu with a right mouse click and select the "Export ASCII (All cells)" option. This allow
- separation_of_2_species_with @howto
- d laser. ===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[pro... separation_of_2_species_with_Image_2.png }} * Select the "Analysis" tab and in there, open the drop do... as evenly splitted onto two detectors. Therefore, select both detectors in the definition of "Channel A": ... ration_of_2_species_with_Image_10.png?383 }} * Select the file ''Cy5_diff_IRF+FLCS-pattern.ptu'' and cl
- intensity_time_trace_analysis @howto
- d open the context menu with a right mouse click. Select "Export ASCII: Selected cell". {{ using_the_intensity_time_trace_script... tion of new setups. * In the sample workspace, select the file ''GUVs.ptu'' and apply the time trace sc... spikes. * Open the pulldown menu "Marker" and select all markers. {{ using_the_intensity_time_trace_sc... ing the context menu with a right mouse click and select "Rescale". * In this way, the marker assignment
- using_the_anisotropy_image_script @howto
- tivate the context menu with a right mouse click, select a ROI selection tool and mark the regions you want to analyze.... _Image_22.png }} **Response:** A window opens to select the user settings file. Select the recently generated file. {{ using_the_anisotr