“[…] PyCorrFit is a general-purpose FCS evaluation software that, amongst other formats, supports the established Zeiss ConfoCor3 ~.fcs file format. PyCorrFit comes with several built-in model functions, covering a wide range of applications in standard confocal FCS. In addition, it contains equations dealing with different excitation geometries like total internal reflection (TIR).”
GitHub:https://github.com/paulmueller/PyCorrFit
PyCorrFit does support the PicoQuant t3r file format (currently only '*.pt3') https://github.com/paulmueller/PyCorrFit/issues/69
An enhancement request for implementing '*.ptu' file format support is existing. https://github.com/paulmueller/PyCorrFit/issues/84
PyCorrFit is described in: Müller, P., Schwille, P., and Weidemann, T. PyCorrFit - generic data evaluation for fluorescence correlation spectroscopy. Bioinformatics 2014. http://dx.doi.org/10.1093/bioinformatics/btu328