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- flim_fret_calculation_for_multi_exponential_donors
- ere might be slight differences compared to other software versions. For example the way of setting an inten... the FLIM analysis ==== * Start SymPhoTime 64 software. * Open the "Samples" workspace via "File\open ... iexpd_reconvfit_response.png |}} **Note:**\\ The software offers the possibility to fit the data using a n-... o:flim-fret-multiexpd_initialfit.png |}} * The software calculates the amplitude weighted average lifetim
- calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script
- * Start [[products:SymPhoTime64|SymPhoTime 64]] software. * Open the "Samples" workspace via "File\open ... nse:** A window pops up with the message that the software needs to be restarted to apply changes. Press OK.... he_pie-fret_script_Image_18.png }} **Response:** Software restarts, but applies the user profile ''PIE-FRET... nse:** A window pops up with the message that the software needs to be restarted to apply changes. Press OK.
- symphotime_tips_and_tricks
- hoTime64]] that can help to make working with the software more comfortable. In detail, this tutorial covers... version 2.5 </WRAP>|<WRAP> this file type is for software internal purposes only, so the user does not need... uired with a scanner controlled by the SymPhoTime software or the size info has been entered before the meas... ems, which are not remotely controlled by the LSM software via a handshake, the image size is not known unle
- antibunching_measurements
- ducts:HydraHarp 400]] softwares as well as with [[software:SymPhoTime64]], where the sync divider can be dis... -mode]] *measurements are possible with [[software:SymPhoTime64]] ==== Changing from Normal Acquisi... ver, it's enough to disable the SYNC in the SEPIA software => no cable change necessary. === Software Settings during Acquisition === [{{ howto:100210_06_antibunc
- phasor_analysis
- t bin_export analysis tutorial SymPhoTime Globals software}} ~~TOC~~ ====== Phasor Analysis ====== Phas... ing PicoQuant BIN files]] tutorial of the Globals Software package to import the BIN files. * follow the [... is.pdf| Phasor Analysis Tutorial]] of the Globals Software. * [[https://download.lfd.uci.edu/tutorials/... time FCS]] Additional Tutorials for the Globals software can be found here: [[http://www.lfd.uci.edu/globa
- registering_new_scripts
- be generated and registered in the SymPhoTime 64 software. Therefore, a present SymPhoTime 64 demo script "... * Start [[products:SymPhoTime64|SymPhoTime 64]] software. * Go to the "Analysis" tab. * Start the scri... nes the name of the script used in the SymPhoTime software. * In the main menu of the window, select "File... ext "changes will take effect after restating the software". * Restart SymPhoTime 64. **Response:** Under
- howto
- kspace downloadable [[http://www.picoquant.com/dl_software/SPT64/Demo_workspace_SPT64.zip|here]] (1.5GB). =... /fluorescence-spectrometers/easytau-2|EasyTau 2]] software are directly stored in a workspace in ''.etc'' da... utton. * It is also possible to use the native software of the timing electronics for data acquisition, i... ore the histograms in ''.phu'' data format. Older software versions of various timing electronics may use ot
- visualizing_dynamics_using_the_multiframe-flim_script
- start the analysis ==== * Start SymPhoTime 64 software (this tutorial uses version 2.5). * Open the "T... n the fitting parameter table.\\ **Note:**\\ The software offers the possibility to fit the data using a n-... files, which can be opened e..g using open source software like Fiji\\ - BIN exports the image series as a... ge size information automatically via a handshake software, as in several LSMs, the image size is automatica
- calculate_ratiometric_single_pair_fret_distributions
- * Start [[products:SymPhoTime64|SymPhoTime 64]] software. * Open the "Samples" workspace via "File\open ... nse:** A window pops up with the message that the software needs to be restarted to apply changes. Press OK.... fret_distributions_Image_20.png }} **Response:** Software restarts, but applies the user profile ''SM-FRET.
- data_file_import
- nstallation of the [[https://www.picoquant.com/dl_software/TimeHarp260/TimeHarp260_SW_and_DLL_V3_2.zip|native TimeHarp260 software]]. This file contains two histograms: an [[howto:... g. The same data file, when opened in the native software, looks like this: {{ :howto:et2_originalhistogra
- update
- start the analysis ==== * Start SymPhoTime 64 software (this tutorial uses version 2.5, there might be d... { howto:addroi.png?400 |}} **Response:**\\ The software assigns sequential numbers to selected ROIs.The w... n the fitting parameter table.\\ **Note:**\\ The software offers the possibility to fit the data using an n
- using_the_anisotropy_image_script
- * Start [[products:SymPhoTime64|SymPhoTime 64]] software. * Open the "Samples" workspace via "File\open ... nse:** A window pops up with the message that the software needs to be restarted to apply changes. Press OK.... tropy_image_script_Image_24.png }} **Response:** Software restarts, but applies the user profile ''Anisotro
- calculate_ratiometric_fret-images
- * Start [[products:SymPhoTime64|SymPhoTime 64]] software.\\ * Open the "Samples" workspace via "File\ope... main menu ("Settings\Save User Profile as"). The software will need to restart to adapt the changes. * If
- calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script
- * Start [[products:SymPhoTime64|SymPhoTime 64]] software. * Open the "Samples" workspace via "File\open ... user profile need to be confirmed whenclosing the software. A message will pop up when closing SymPhoTime 64
- determination_of_the_focal_width_with_the_focal
- * Start [[products:SymPhoTime64|SymPhoTime 64]] software. * Open the "Samples" workspace via "File\open ... ed with a scanner controlled by the SymPhoTime 64 software (i.e. the piezo scanner in the [[products:MicroTi