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- flim_fret_calculation_for_multi_exponential_donors @howto
- rescence is detected, is randomly spread over the TCSPC window, the mean photon arrival time of the dark ... background is usually very long (up to 1/2 of the TCSPC window), which makes the color scale loaded by de... g is then applied to the lifetime graph(s) in the TCSPC histogram.\\ **Lower center/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (=
- lifetime_fitting_using_the_tcpsc_fitting_script @howto
- lysis" tab and in there, open the drop down menu "TCSPC". {{ lifetime_fitting_using_the_tcpsc_fitting_scr... op down menu: {{grey_button.png}} * Start the "TCSPC Fitting" script by clicking on "Start". {{ lifeti... _fitting_script_Image_4.png }} **Response:** The TCSPC Fitting script is applied to the file ''ATTO655_d... **Center/right:** TCPSC fitting graph showing the TCSPC histogram of the measured sample. * In the de
- lifetime-fitting_using_the_flim_script @howto
- rescence is detected, is randomly spread over the TCSPC window, the mean photon arrival time of the dark ... ually very long (with an average up to 1/2 of the TCSPC window), which makes the color scale loaded by de... lifetime graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\ **5: [[glossary:TCSPC]] Decay window**\\ By default, this set shows the [[glossary:TCSP
- tcspc_external_markers @support
- {{tag>TCSPC scanner markers TTTR Harps PH300 HH400 TH260 SymPhoTime64}} ~~TOC~~ ====== TCSPC modules and External Markers====== Here a general... rkers from a controller of scanning module to the TCSPC module. The markers are incorporated into the raw data according to the principles of [[http://tcspc.com/doku.php/glossary:tttr|Time Tagged Time Resol
- lifetime_fitting_using_the_flim_analysis @howto
- rescence is detected, is randomly spread over the TCSPC window, the mean photon arrival time of the dark ... background is usually very long (up to 1/2 of the TCSPC window), which makes the color scale loaded by de... ied to the lifetime graph(s) on the lower center (TCSPC histogram).\\ **Lower center/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (=
- roi_fitting_using_the_flim_script @howto
- rescence is detected, is randomly spread over the TCSPC window, the mean photon arrival time of the dark ... background is usually very long (up to 1/2 of the TCSPC window), which makes the color scale loaded by de... ied to the lifetime graph(s) on the lower center (TCSPC histogram).\\ - **Lower center/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (=
- visualizing_dynamics_using_the_multiframe-flim_script @howto
- rescence is detected, is randomly spread over the TCSPC window, the mean photon arrival time of the dark ... ually very long (with an average up to 1/2 of the TCSPC window), which makes the color scale loaded by de... lifetime graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\ **5: [[glossary:TCSPC]] Decay window**\\ By default, this set shows the [[glossary:TCSP
- software
- are the maintainer of a software that deals with TCSPC data please edit this page and add your software ... me Correlated Single Photon Counting Instruments (TCSPC devices). It harnesses the advantages of C++ for ... een the high-speed capabilities of your PicoQuant TCSPC device and the ease of use and versatility of Pyt... o analyze time-correlated single-photon counting (TCSPC) data sets, namely fluorescence lifetime and time
- separation_of_2_species_with @howto
- fluorescence lifetime measurements via [[glossary:tcspc|TCSPC]], this script can only be applied, if at least one fluorophore is excited with a pulsed laser. =... nents are available, it is possible to load these TCSPC histograms as patterns for the filter definition.... ttern Matching" approach. * If the individual TCSPC histograms are not available, or a dye is analyze
- update @howto
- rescence is detected, is randomly spread over the TCSPC window, the mean photon arrival time of the dark ... ually very long (with an average up to 1/2 of the TCSPC window), which makes the color scale loaded by de... lifetime graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\ **5: [[glossary:TCSPC]] Decay window**\\ By default, this set shows the [[glossary:TCSP
- lifetime-fitting_using_the_rapid_reconvolution_algorithm @howto
- rescence is detected, is randomly spread over the TCSPC window, the mean photon arrival time of the dark ... ually very long (with an average up to 1/2 of the TCSPC window), which makes the color scale loaded by de... lifetime graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\ **5: [[glossary:TCSPC]] Decay window**\\ By default, this set shows the [[glossary:TCSP
- how_to_work_with_the_instrument_response_function_irf @howto
- re possible which are described here: https://www.tcspc.com/doku.php/howto:how_to_measure_the_instrument_... has been stopped, when the decay histogram in the TCSPC preview has reached ~10000 counts in the peak cha... 57fcfa684daef86c23]].\\ 2. In Analysis tab, under TCSPC, find `TCSPC Decay`. Click Start; {{ howto:tcspc_decay_gui.png?400 |}} Response: you see this kind of c
- symphotime_tips_and_tricks @howto
- opens with a preview display (Image, FCS-traces, TCSPC-curve or Time Trace - depending on the type of an... Time-Trace === * Max. Counts * Av. Counts === TCSPC === * Max. Counts (number of photons in the peak TCSPC-channel) You can also add additional information... ts can be set (e.g. 0 as minimum lifetime) in the TCSPC fitting panel * the fast lifetime in an ideal s
- pile-up_effect @glossary
- {{tag> TCSPC pile-up dead_time}} ~~TOC~~ ====== Pile-Up Effect ====== The Pile-Up effect describes the e... gh photon count rates due to the dead time of the TCSPC devices. Most single photon counting detectors and [[TCSPC]] electronics have a [[dead time]]. After registe... for the next photon to detect. The dead time for TCSPC devices used for the measurement of fluorescence
- calculate_fccs_trace_with_the_grouped_fcs_script @howto
- :** In green, the selected area of the [[glossary:tcspc|TCSPC]] window for FCS calculation is marked. It is calculated on the assumption that the sample is exci... ase in our sample, we have to select the complete TCSPC window to avoid loosing fluorescence photons. ... r 1), move the red bar to the right to expand the TCSPC selection over the whole TCSPC window for that ch