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- flim_fret_calculation_for_multi_exponential_donors
- rescence is detected, is randomly spread over the TCSPC window, the mean photon arrival time of the dark ... background is usually very long (up to 1/2 of the TCSPC window), which makes the color scale loaded by de... g is then applied to the lifetime graph(s) in the TCSPC histogram.\\ **Lower center/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (=
- lifetime_fitting_using_the_tcpsc_fitting_script
- lysis" tab and in there, open the drop down menu "TCSPC". {{ lifetime_fitting_using_the_tcpsc_fitting_scr... op down menu: {{grey_button.png}} * Start the "TCSPC Fitting" script by clicking on "Start". {{ lifeti... _fitting_script_Image_4.png }} **Response:** The TCSPC Fitting script is applied to the file ''ATTO655_d... **Center/right:** TCPSC fitting graph showing the TCSPC histogram of the measured sample. * In the de
- lifetime-fitting_using_the_flim_script
- rescence is detected, is randomly spread over the TCSPC window, the mean photon arrival time of the dark ... ually very long (with an average up to 1/2 of the TCSPC window), which makes the color scale loaded by de... lifetime graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\ **5: [[glossary:TCSPC]] Decay window**\\ By default, this set shows the [[glossary:TCSP
- lifetime_fitting_using_the_flim_analysis
- rescence is detected, is randomly spread over the TCSPC window, the mean photon arrival time of the dark ... background is usually very long (up to 1/2 of the TCSPC window), which makes the color scale loaded by de... ied to the lifetime graph(s) on the lower center (TCSPC histogram).\\ **Lower center/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (=
- roi_fitting_using_the_flim_script
- rescence is detected, is randomly spread over the TCSPC window, the mean photon arrival time of the dark ... background is usually very long (up to 1/2 of the TCSPC window), which makes the color scale loaded by de... ied to the lifetime graph(s) on the lower center (TCSPC histogram).\\ - **Lower center/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (=
- visualizing_dynamics_using_the_multiframe-flim_script
- rescence is detected, is randomly spread over the TCSPC window, the mean photon arrival time of the dark ... ually very long (with an average up to 1/2 of the TCSPC window), which makes the color scale loaded by de... lifetime graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\ **5: [[glossary:TCSPC]] Decay window**\\ By default, this set shows the [[glossary:TCSP
- separation_of_2_species_with
- fluorescence lifetime measurements via [[glossary:tcspc|TCSPC]], this script can only be applied, if at least one fluorophore is excited with a pulsed laser. =... nents are available, it is possible to load these TCSPC histograms as patterns for the filter definition.... ttern Matching" approach. * If the individual TCSPC histograms are not available, or a dye is analyze
- update
- rescence is detected, is randomly spread over the TCSPC window, the mean photon arrival time of the dark ... ually very long (with an average up to 1/2 of the TCSPC window), which makes the color scale loaded by de... lifetime graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\ **5: [[glossary:TCSPC]] Decay window**\\ By default, this set shows the [[glossary:TCSP
- lifetime-fitting_using_the_rapid_reconvolution_algorithm
- rescence is detected, is randomly spread over the TCSPC window, the mean photon arrival time of the dark ... ually very long (with an average up to 1/2 of the TCSPC window), which makes the color scale loaded by de... lifetime graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\ **5: [[glossary:TCSPC]] Decay window**\\ By default, this set shows the [[glossary:TCSP
- how_to_work_with_the_instrument_response_function_irf
- re possible which are described here: https://www.tcspc.com/doku.php/howto:how_to_measure_the_instrument_... has been stopped, when the decay histogram in the TCSPC preview has reached ~10000 counts in the peak cha... 57fcfa684daef86c23]].\\ 2. In Analysis tab, under TCSPC, find `TCSPC Decay`. Click Start; {{ howto:tcspc_decay_gui.png?400 |}} Response: you see this kind of c
- symphotime_tips_and_tricks
- opens with a preview display (Image, FCS-traces, TCSPC-curve or Time Trace - depending on the type of an... Time-Trace === * Max. Counts * Av. Counts === TCSPC === * Max. Counts (number of photons in the peak TCSPC-channel) You can also add additional information... ts can be set (e.g. 0 as minimum lifetime) in the TCSPC fitting panel * the fast lifetime in an ideal s
- calculate_fccs_trace_with_the_grouped_fcs_script
- :** In green, the selected area of the [[glossary:tcspc|TCSPC]] window for FCS calculation is marked. It is calculated on the assumption that the sample is exci... ase in our sample, we have to select the complete TCSPC window to avoid loosing fluorescence photons. ... r 1), move the red bar to the right to expand the TCSPC selection over the whole TCSPC window for that ch
- visualizing_dynamics_with_the_multi_frame_flim_analysis
- detected, is randomly spread over the [[glossary:TCSPC]] window, the mean photon arrival time of the dar... is usually very long (up to 1/2 of the [[glossary:TCSPC]] window), which makes the color scale loaded by ... pplied to the lifetime graph(s) in the [[glossary:TCSPC]] histogram.\\ - **Lower center/left:** [[glossary:TCSPC]] histogram of all photons in the image in green
- flim-fret_calculation_for_single_exponential_donors
- rescence is detected, is randomly spread over the TCSPC window, the mean photon arrival time of the dark ... background is usually very long (up to 1/2 of the TCSPC window), which makes the color scale loaded by de... onential decay kinetics. - **Lower center/left: TCSPC histogram of all photons in the image.** In green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1)
- using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs
- uous wave laser, the fluorescence response in the TCSPC window will be a homogenous background similar to... cence response after cw excitation.\\ After these TCSPC patterns have been generated, the green spectral ... lccs_Image_10.png?353 }} **Response:** * The TCSPC histogram for this file appears in the "Pattern" ... nd" as active filter, and move the red bar in the TCSPC window to the right to highlight the complete TCS