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fluorescence_correlation_spectroscopy-_a_short_introduction
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{{tag>MicroTime LSM Theory SymPhoTime FCS Correlation}} ====== Introduction to Fluorescence Correlation Spectroscopy... opulation of freely diffusing molecules. In short time, the theoritical framework of FCS was expanded to... udy of rotation diffusion. Unfortunately, at that time the application of FCS was impractical due to the
advantages_and_disadvantages_of_two_photon_excitation_tpe
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broader so that many dyes are excited at the same time. This is an advantage when autofluorescence is in... order of magnitude smaller: You will get just ten times less fluorescence photons. This leads to limitat... 0 MHz, which in turn give you only an observation time of 12.5 ns in the life time of the dyes. This is too short for most of the dyes. With pulse pickers the
community_news
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performed FCS measurements with PicoQuant's MicroTime 200 microscope to probe the mobility of proteins ... s. Also impressive, they use PicoQuant's 6th MicroTime 200 microscope, built in 2004, for performing thi... approach of **Molecular Tension-Fluorescence Lifetime Imaging Microscopy (MT-FLIM)** \\ \\ * **24.10.