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- t3-mode
- === T3-mode ====== (derived from [[tttr]]-mode or Time-Tagged-Time-Resolved mode) A special measurement mode of the [[products:PicoHarp 300]], [[products:Hyd... reduces the sync rate so that the channel [[dead time]] is no longer a problem. * The remaining prob... vent and the last sync event, and - the arrival time of the event pair on the overall experiment time
- t2-mode
- on about the channel it came from and the arrival time of the event with respect to the overall measurem... 4 bits for the channel number and 28 bits for the time-tag. Routing is not supported. If the time tag overflows, a special overflow marker record is inserted ... ssing of the data stream a theoretically infinite time span can be recovered at full resolution. [[Dead
- pile-up_effect
- {{tag> TCSPC pile-up dead_time}} ~~TOC~~ ====== Pile-Up Effect ====== The Pile-Up effect describes the ... s lost at high photon count rates due to the dead time of the TCSPC devices. Most single photon counti... detectors and [[TCSPC]] electronics have a [[dead time]]. After registering one photon the device needs some time to be ready for the next photon to detect. The de
- bifl
- **B**urst **I**ntegrated **F**luorescence **L**ifetime: The photon events of a complete burst are accumu... into a [[TCSPC]] histogram which is used for lifetime analysis methods afterwards. Usually a burst is defined as a time interval for which the intensity of a time trace or [[MCS]] measurement is above a chosen threshold. Ev
- differential_count_rate
- {{tag> pile-up dead_time IRF differential_countrate}} ====== Differential Count Rate ====== Ideally, a... y (probability of encountering a photon) during a time interval when a detected signal is really present... the detector bunched, as flashes. These are short time intervals with huge photon density (rate), separa... rate" is a result of **sparse sampling with dead time**. The sampled signal features much higher photon
- fast_lifetime
- ====== Fast lifetime ====== A method for estimating the average lifetime, calculated non-distinctive on different exponenti... used with [[FLIM]] go get a first idea of the lifetime distribution in the image. In detail, FastLT is ... the barycentre of the (pseudo–)pixel's decay. The time span from the barycentre of the IRF to the baryce
- tttr
- ====== TTTR ====== TTTR stands for Time-Tagged Time-Resolved. The basic concept of this measurement mode is to record every individual photon of a [[TCSPC]] experiment. Both the arrival time as well as the TCSPC timing information (i.e. the time from the preceding excitation pulse or to the fol
- dead_time
- ====== Dead Time ====== In TCSPC the term dead time refers to the time the TCSPC system needs armed again after detecting an event. During the dead time the TCSPC system is blind. If for example two p
- mcs
- surement are sorted by means of their macroscopic time into equally spaced time bins. What immediately results is a time trace of the fluorescence intensity. In a subsequent analysis ... form [[TCSPC]] histograms, which in turn can be used for lifetime analysis, time-gated analysis etc.
- flim
- " ===== FLIM stands for **F**luorescence **L**ifetime **IM**aging or **F**luorescence **LI**fetime **M**icroscopy. ([[wp>Fluorescence-lifetime imaging microscopy]]). In FLIM additional image contrast t... used to visualize the average [[fluorescence lifetime]] in each pixel of the image. ===== FLIM on this
- marquardt-levenberg
- sed in our [[software:FluoFit]], [[software:SymPhoTime]] and a variety of other products (online-Fitting... traight-forward manner). Especially for the SymPhoTime software this is a severe limitation, since least... in [[FLIM]] measurements as analysed by the SymPhoTime software the number of photons for a given decay ... ]] fitting. When running a [[MLE]] fit the SymPhoTime software abandons the ML-fitting and switches to
- deconvolution
- == Deconvolution ====== Deconvolution is used in time domain data analysis for removal of broadening ef... nvolution, the best way of doing it (at least for time domain data) would be via Fourier transform. Four... or deconvolution-like) techniques. What holds for time domain data, also holds for imaging: Deconvolutio
- fast_flim
- FLIM ====== [[FLIM]] image, using the [[fast lifetime]] calculation. The color-coded fast lifetime of each pixel represents the average photon arrival time with respect to the preceding laser pulse. =====
- tcspc
- ed into histogram channels that correspond to the time that elapsed since the last excitation pulse. It is a time domain method for investigating time resolved fluorescence.
- bootstrap
- d fitted, the bootstrap method seems to be rather time consuming. On the other hand all parameter errors... one go"; also the simulation process is not very time consuming. The bootstrap method makes far less as