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- intensity_time_trace_analysis
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- lifetime_fitting_using_the_flim_analysis
- {{tag>howto analysis FLIM imaging tutorial SymPhoTime}} ~~NOTOC~~ ====== Lifetime Fitting Using the FLIM Analysis ====== ===== Summary ===== This tuto... hows step-by-step, how the FLIM analysis of SymPhoTime 64 can be used to perform a lifetime fit and how to extract and read the results. In detail, the FLIM a
- flim_fret_calculation_for_multi_exponential_donors
- g>tutorial howto analysis FLIM imaging FRET SymPhoTime}} ~~NOTOC~~ ====== FLIM-FRET Calculation for M... This tutorial shows step-by-step, how the "Lifetime FRET Image" analysis of SymPhoTime 64 can be used to calculate pixel-by-pixel the average [[glossary:fr... average FRET-efficiency, first the donor only lifetime is determined using cells that have only been tra
- lifetime-fitting_using_the_rapid_reconvolution_algorithm
- {{tag>howto tutorial FLIM analysis SymPhoTime MT LSM_upgrade reconvolution fitting}} ====== Lifetime-Fitting Using the Rapid Reconvolution Model =====... IM]]. Here in detail the FLIM analysis of SymPhoTime 64 is used to perform a lifetime fit and how to extract and read the results. A mixture of Dragon Green
- intensity_time_trace_analysis
- {{tag> tutorial howto time-trace analysis SymPhoTime}} ~~NOTOC~~ ======Intensity Time Trace Analysis====== ===== Summary ===== This tutorial shows step-by-step, how to draw intensity time traces from a single molecule. The script require
- lifetime_fitting_using_the_tcpsc_fitting_script
- {{tag>howto analysis lifetime tutorial SymPhoTime}} ~~NOTOC~~ ====== SymPhoTime Lifetime Fitting ====== ===== Summary ===== This tutorial shows step-by-step, how to fit a selecte
- lifetime-fitting_using_the_flim_script
- {{tag>howto tutorial FLIM analysis SymPhoTime MicroTime LSM_upgrade}} ====== Lifetime-Fitting Using the FLIM Analysis (updated for SymPhoTime V 2.5 and above) ====== ===== Introduction ====
- flim-fret_calculation_for_single_exponential_donors
- g>tutorial howto FRET FLIM imaging analysis SymPhoTime}} ~~NOTOC~~ ====== FLIM-FRET Calculation for S... This tutorial shows step-by-step, how the "Lifetime FRET Image" analysis of [[products:SymPhoTime64|SymPhoTime 64]] can be used to calculate pixel-by-pixel the FRET efficiency in an image containing a
- visualizing_dynamics_using_the_multiframe-flim_script
- ging analysis dynamics tutorial LSM_upgrade SymPhoTime}} ====== Visualizing Dynamics Using the Multi Frame FLIM Analysis (updated for SymPhoTime v2.5 and above) ====== ===== Introduction ===== ... age containing many frames is treated here like a time-series. The example image shows the plasma membra... rane tension changes through its fluorescence lifetime changes. This has been published 2018 in nature:
- roi_fitting_using_the_flim_script
- g> howto FLIM imaging ROI analysis tutorial SymPhoTime}} ~~NOTOC~~ ====== ROI Fitting Using the FLIM A... [[glossary:flim|FLIM]] script of [[products:SymPhoTime64|SymPhoTime 64]] can be used to fit several regions of interest (ROIs), and how to extract and interpr... start the script ==== * Start [[products:SymPhoTime64|SymPhoTime 64]] software. * Open the "Samples
- update
- {{tag>howto tutorial FLIM analysis SymPhoTime MT LSM_upgrade}} ====== ROI Fitting Using the FLIM Analysis (updated for SymPhoTime v2.5)====== ===== Introduction ===== This tutoria... shows step-by-step, how the FLIM script of SymPhoTime 64 can be used to fit several regions of interest... file and start the analysis ==== * Start SymPhoTime 64 software (this tutorial uses version 2.5, ther
- visualizing_dynamics_with_the_multi_frame_flim_analysis
- ti-frame imaging analysis dynamics tutorial SymPhoTime}} ~~NOTOC~~ ====== Visualizing Dynamics Using t... start the script ==== * Start [[products:SymPhoTime64|SymPhoTime 64]] software. * Open the "Samples" workspace via "File -> Open Workspace" from the main... e "Samples" workspace is delivered with the SymPhoTime 64 and on the CD-ROM and contains example data to
- symphotime_tips_and_tricks
- {{tag>Howto Analysis Tutorial SymPhoTime Tips&Tricks}} ====== SymPhoTime 64 Analysis Tips and Tricks ====== ~~TOC~~ ===== Summary ===== Th... orial explains a few features of [[products:SymPhoTime64]] that can help to make working with the softwa... * Different file-types used or generated by SymPhoTime64 * Image Display * ROI-handling * Export
- separation_of_2_species_with
- {{tag> howto tutorial FCS FLCS analysis SymPhoTime}} ~~NOTOC~~ ====== Separation of 2 Species with Dif... . cis/trans isomerization.\\ The fluorescence lifetime of both dyes differ significantly (~1.8 ns for AT... 655 and ~1 ns for Cy5). In FLCS (Fluorescence Lifetime Correlation Spectroscopy), the lifetimes are used... calculate FCS autocorrelation curves for each lifetime component. The aim of this script is to demonstra
- calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script
- {{tag> tutorial howto FRET PIE analysis time-trace SymPhoTime}} ~~NOTOC~~ ====== Calculate Ratiometric Single Pair FRET Distributions Using PIE-FRE... cript requires a spectrally resolved fluorescence time trace, in which donor and acceptor have been exci... gJpZhrY?large }} **Note:** The script requires a time trace containing the fluorescence of two spectral
- how_to_measure_the_instrument_response_function_irf
- ved. Such a cocktail cannot be stored for a long time. Latest the KI will photochemically decompose. =... of course) any of the above mentioned fast decay time fluorophores and record their response. The signa... surement series, provided that the system has had time to warm up (~15min), and neither the repetition r... used as for the sample), and re-used later. Over time or upon changes of the excitation wavelength, the