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- fluorescence_correlation_spectroscopy-_a_short_introduction
- opulation of freely diffusing molecules. In short time, the theoritical framework of FCS was expanded to... udy of rotation diffusion. Unfortunately, at that time the application of FCS was impractical due to the... e mean product of the fluorescence intensity F at time t with the fluuorescence intensity after a lag time τ divided by the mean fluorescence intensity square
- advantages_and_disadvantages_of_two_photon_excitation_tpe
- broader so that many dyes are excited at the same time. This is an advantage when autofluorescence is in... order of magnitude smaller: You will get just ten times less fluorescence photons. This leads to limitat... 0 MHz, which in turn give you only an observation time of 12.5 ns in the life time of the dyes. This is too short for most of the dyes. With pulse pickers the